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Angiopoietin 1 Monoclonal antibody

Angiopoietin 1 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

3C4A3

Cat No : 68618-1-Ig

Print datasheet

Synonyms

AGP1, AGPT, ANG 1, ANG1, angiopoietin 1, ANGPT1, KIAA0003

验证数据展示

  • WB analysis using 68618-1-Ig

    WB analysis using 68618-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68618-1-Ig (ANGPT1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inA375 cells, PNGase F treated A375 cells, non-treated U-87 MG cells, PNGase F treated U-87 MG cells, non-treated HuH-7 cells, PNGase F treated HuH-7 MG cells, non-treated bENd.3 cells, PNGase F treated bENd.3 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68618-1-Ig targets Angiopoietin 1 in WB, ELISA applications and shows reactivity with Human, mouse samples.

Tested Applications WB, ELISA
Tested Reactivity Human, mouse
Immunogen Angiopoietin 1 fusion protein Ag18829 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name angiopoietin 1
Synonyms AGP1, AGPT, ANG 1, ANG1, angiopoietin 1, ANGPT1, KIAA0003
Calculated Molecular Weight 498 aa, 58 kDa
Observed Molecular Weight70 kDa, 55 kDa
GenBank Accession NumberBC152411
Gene Symbol ANGPT1
Gene ID (NCBI) 284
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ15389
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Angiopoietin 1 is a 70-kDa secreted glycoprotein generated from vascular mural cells, pericytes, and certain other cells. Angiopoietin 1 participates in vascular differentiation through angiogenesis, which is the process of the growth and remodelling of existing vessels. Angiopoietin 1 is also involved in the maintenance and turnover of blood vessels in mature animals. Angiopoietin 1 binds to and activates the TEK/TIE2 receptor by inducing its dimerization and tyrosine phosphorylation, playing an important role in the regulation of angiogenesis. Overexpression of Angiopoietin 1 has been proven to occur in malignant glioblastoma, neuroblastoma, non-small cell lung cancer, and other tumors.

实验方案

Product Specific Protocols
WB protocol for Angiopoietin 1 antibody 68618-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols