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验证数据展示

  • Dot Blot experiment of / using 86768-1-RR

    Dot Blot experiment of / using 86768-1-RR

    Dot blot analysis was used to confirm the specificity of 86768-1-RR Acetyl-Histone H4 (Lys91) antibody. peptides were spotted onto NC and probed with antibody at 1 µg/ml.The amount of peptide (μg/mL) spotted is indicated next to each row.

  • ChIP experiment of HeLa using 86768-1-RR

    ChIP experiment of HeLa using 86768-1-RR

    Chromatin was prepared from HeLa cells. Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 15 µg of cross-linked chromatin, 5 µg of Acetyl-Histone H4 (Lys91) (86768-1-RR) or 5 ug of Normal Rabbit IgG (98136-1-RR), and 20 µl of Protein A Magarose Beads. The immunoprecipitated DNA was quantified by real-time PCR.

  • WB analysis of NIH/3T3 using 86768-1-RR

    WB analysis of NIH/3T3 using 86768-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 86768-1-RR (Acetyl-Histone H4 (Lys91) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of HSC-T6 using 86768-1-RR

    WB analysis of HSC-T6 using 86768-1-RR

    Trichostatin A treated HSC-T6 cells were subjected to SDS PAGE followed by western blot with 86768-1-RR (Acetyl-Histone H4 (Lys91) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 86768-1-RR

    IF Staining of HeLa using 86768-1-RR

    Immunofluorescent analysis of (4% PFA) fixed Trichostatin A treated HeLa cells using Acetyl-Histone H4 (Lys91) antibody (86768-1-RR, Clone: 251157D9 ) at dilution of 1:2000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • FC experiment of NIH/3T3 using 86768-1-RR

    FC experiment of NIH/3T3 using 86768-1-RR

    1x10^6 Trichostatin A treated NIH/3T3 cells were intracellularly stained with 0.13 ug Acetyl-Histone H4 (Lys91) Recombinant monoclonal antibody (86768-1-RR, Clone:251157D9) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.13 ug Isotype Control (blue). Cells were fixed and permeabilized with Flow Cytometry Phosphorylated Protein Fixation/Permeabilization Kit (PF00026).

Acetyl-Histone H4 (Lys91) Recombinant monoclonal antibody

Acetyl-Histone H4 (Lys91) Uni-rAb® Recombinant Antibody for WB, IF/ICC, FC (Intra), Dot Blot, ELISA, ChIP-qPCR
Cat No. 86768-1-RR

产品说明书

CloneNo. 251157D9

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, FC (Intra), Dot Blot, ELISA, ChIP-qPCR

别名

Ac Histone H4, Ac Histone H4 (Lys91), Ac Histone H4 Lys91, Ac Histone H4-Lys91, Acetyl Histone

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inTrichostatin A treated NIH/3T3 cells, Trichostatin A treated HSC-T6 cells
Positive IF/ICC detected inTrichostatin A treated HeLa cells
Positive FC (Intra) detected inTrichostatin A treated NIH/3T3 cells
Positive Dot Blot detected in/ /
Positive ChIP-qPCR detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:1000-1:4000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.13 ug per 10^6 cells in a 100 µl suspension
DOT BLOTDOT BLOT : 1:10-1:100
CHIP-QPCRCHIP-QPCR : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

86768-1-RR targets Acetyl-Histone H4 (Lys91) in WB, IF/ICC, FC (Intra), Dot Blot, ELISA, ChIP-qPCR applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, FC (Intra), Dot Blot, ELISA, ChIP-qPCR Application Description
经测试反应性 human, mouse, rat
免疫原

Peptide

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 histone cluster 1, H4a
别名 Ac Histone H4, Ac Histone H4 (Lys91), Ac Histone H4 Lys91, Ac Histone H4-Lys91, Acetyl Histone
观测分子量15 kDa
GenBank蛋白编号BC069654
基因名称 HIST1H4A
Gene ID (NCBI) 8359
RRIDAB_3745109
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP62805
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Histones are small, highly basic proteins that consist of a globular domain with unstructured N- and C-terminal tails protruding from the main structure. Histone H3 is one of the five main histones that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. In addition to their role in DNA compartmentalization, histones also play crucial roles in various biologic processes, including gene expression and regulation, DNA repair, chromatin condensation, cell cycle progression, chromosome segregation, and apoptosis. The ability of histones to regulate chromatin dynamics primarily originates from various posttranslational modifications carried out by histone-modifying enzymes.

实验方案

Product Specific Protocols
FC protocol for Acetyl-Histone H4 (Lys91) antibody 86768-1-RRDownload protocol
IF protocol for Acetyl-Histone H4 (Lys91) antibody 86768-1-RRDownload protocol
WB protocol for Acetyl-Histone H4 (Lys91) antibody 86768-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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