×
Use Able AI chat for product recommendations

验证数据展示

  • Dot Blot experiment of / using 86768-1-RR (same clone as 86768-1-PBS)

    Dot Blot experiment of / using 86768-1-RR (same clone as 86768-1-PBS)

    Dot blot analysis was used to confirm the specificity of 86768-1-RR Acetyl-Histone H4 (Lys91) antibody. peptides were spotted onto NC and probed with antibody at 1 µg/ml.The amount of peptide (μg/mL) spotted is indicated next to each row. This data was developed using the same antibody clone with 86768-1-PBS in a different storage buffer formulation.

  • ChIP experiment of HeLa using 86768-1-RR (same clone as 86768-1-PBS)

    ChIP experiment of HeLa using 86768-1-RR (same clone as 86768-1-PBS)

    Chromatin was prepared from HeLa cells. Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 15 µg of cross-linked chromatin, 5 µg of Acetyl-Histone H4 (Lys91) (86768-1-RR) or 5 ug of Normal Rabbit IgG (98136-1-RR), and 20 µl of Protein A Magarose Beads. The immunoprecipitated DNA was quantified by real-time PCR. This data was developed using the same antibody clone with 86768-1-PBS in a different storage buffer formulation.

  • WB analysis of NIH/3T3 using 86768-1-RR (same clone as 86768-1-PBS)

    WB analysis of NIH/3T3 using 86768-1-RR (same clone as 86768-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86768-1-RR (Acetyl-Histone H4 (Lys91) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86768-1-PBS in a different storage buffer formulation.

  • WB analysis of HSC-T6 using 86768-1-RR (same clone as 86768-1-PBS)

    WB analysis of HSC-T6 using 86768-1-RR (same clone as 86768-1-PBS)

    Trichostatin A treated HSC-T6 cells were subjected to SDS PAGE followed by western blot with 86768-1-RR (Acetyl-Histone H4 (Lys91) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86768-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 86768-1-RR (same clone as 86768-1-PBS)

    IF Staining of HeLa using 86768-1-RR (same clone as 86768-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Trichostatin A treated HeLa cells using Acetyl-Histone H4 (Lys91) antibody (86768-1-RR, Clone: 251157D9 ) at dilution of 1:2000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 86768-1-PBS in a different storage buffer formulation.

Acetyl-Histone H4 (Lys91) Recombinant monoclonal antibody, PBS Only

Acetyl-Histone H4 (Lys91) Uni-rAb® Recombinant Antibody for WB, IF/ICC, Dot Blot, Indirect ELISA, ChIP-qPCR
Cat No. 86768-1-PBS

产品说明书

CloneNo. 251157D9

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, Dot Blot, Indirect ELISA, ChIP-qPCR

别名

Ac Histone H4, Ac Histone H4 (Lys91), Ac Histone H4 Lys91, Ac Histone H4-Lys91, Acetyl Histone

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

点击咨询售前客服

点击咨询售后客服

产品信息

86768-1-PBS targets Acetyl-Histone H4 (Lys91) in WB, IF/ICC, Dot Blot, Indirect ELISA, ChIP-qPCR applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, Dot Blot, Indirect ELISA, ChIP-qPCR Application Description
经测试反应性 human, mouse, rat
免疫原

Peptide

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 histone cluster 1, H4a
别名 Ac Histone H4, Ac Histone H4 (Lys91), Ac Histone H4 Lys91, Ac Histone H4-Lys91, Acetyl Histone
观测分子量15 kDa
GenBank蛋白编号BC069654
基因名称 HIST1H4A
Gene ID (NCBI) 8359
RRIDAB_3745109
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP62805
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Histones are small, highly basic proteins that consist of a globular domain with unstructured N- and C-terminal tails protruding from the main structure. Histone H3 is one of the five main histones that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. In addition to their role in DNA compartmentalization, histones also play crucial roles in various biologic processes, including gene expression and regulation, DNA repair, chromatin condensation, cell cycle progression, chromosome segregation, and apoptosis. The ability of histones to regulate chromatin dynamics primarily originates from various posttranslational modifications carried out by histone-modifying enzymes.

||
New chat

Able™

正在加载，请稍候...