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验证数据展示

  • Dot Blot experiment of peptide using 83095-1-RR

    Dot Blot experiment of peptide using 83095-1-RR

    Dot blot analysis was used to confirm the specificity of Histone H4AK12ac antibody. Acetylated peptides were spotted onto NC and probed with antibody at 1 µg/ml.The amount of peptide (μg/mL) spotted is indicated next to each row. Column 1: H4AK12ac. Column 2: Unmodified H4AK12. Column 3: H4AK5ac. Column 4: Unmodified H4AK5. Column 5: H4AK8Ac. Column 6: Unmodified H4AK8. Column 7: H4AK16ac. Column 8:Unmodified H4AK16. Column 9: H4AK91ac. Column 10: Unmodified H4K91 Column 11: Blank(PBS).

  • WB analysis of NIH/3T3 using 83095-1-RR

    WB analysis of NIH/3T3 using 83095-1-RR

    Untreated and trichostatin A treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 83095-1-RR (Acetyl-Histone H4 (Lys12) antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with Beta Actin Monoclonal antibody (66009-1-Ig) as loading control.

  • WB analysis of HEK-293 using 83095-1-RR

    WB analysis of HEK-293 using 83095-1-RR

    WB result of Acetyl-Histone H4 (Lys12) antibody (83095-1-RR; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Acetyl-Histone H4 (Lys12) transfected HEK-293 cells.

  • WB analysis of HSC-T6 using 83095-1-RR

    WB analysis of HSC-T6 using 83095-1-RR

    HSC-T6 cells were subjected to SDS PAGE followed by western blot with 83095-1-RR (Acetyl-Histone H4 (Lys12) antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours.

  • WB analysis of NIH/3T3 using 83095-1-RR

    WB analysis of NIH/3T3 using 83095-1-RR

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 83095-1-RR (Acetyl-Histone H4 (Lys12) antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse small intestine using 83095-1-RR

    IHC staining of mouse small intestine using 83095-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 83095-1-RR (Acetyl-Histone H4 (Lys12) antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse small intestine using 83095-1-RR

    IHC staining of mouse small intestine using 83095-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 83095-1-RR (Acetyl-Histone H4 (Lys12) antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of Caco-2 using 83095-1-RR

    IF Staining of Caco-2 using 83095-1-RR

    Immunofluorescent analysis of (4% PFA) fixed Caco-2 cells using Acetyl-Histone H4 (Lys12) antibody (83095-1-RR, Clone: 1D18 ) at dilution of 1:300 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • IF Staining of Caco-2 using 83095-1-RR

    IF Staining of Caco-2 using 83095-1-RR

    Immunofluorescent analysis of (4% PFA) fixed Caco-2 cells using Acetyl-Histone H4 (Lys12) antibody (83095-1-RR, Clone: 1D18 ) at dilution of 1:500 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11, red).

  • Featured Product
  • KD/KO验证

Acetyl-Histone H4 (Lys12) Recombinant antibody

Acetyl-Histone H4 (Lys12) Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, Dot Blot, ELISA
Cat No. 83095-1-RR

产品说明书

CloneNo. 1D18

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, Dot Blot, ELISA

别名

Ac Histone H4, Ac Histone H4 (Lys12), Ac Histone H4 Lys12, Ac Histone H4-Lys12, Acetyl Histone

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inTrichostatin A treated NIH/3T3 cells, HEK-293 cells, HSC-T6 cells, NIH/3T3 cells
Positive IHC detected inmouse small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inCaco-2 cells
Positive Dot Blot detected inpeptide

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:150-1:600
DOT BLOTDOT BLOT : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83095-1-RR targets Acetyl-Histone H4 (Lys12) in WB, IHC, IF/ICC, Dot Blot, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, Dot Blot, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 histone cluster 1, H4a
别名 Ac Histone H4, Ac Histone H4 (Lys12), Ac Histone H4 Lys12, Ac Histone H4-Lys12, Acetyl Histone
观测分子量12 kDa
GenBank蛋白编号BC069654
基因名称 HIST1H4A
Gene ID (NCBI) 8359
RRIDAB_3670809
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP62805
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Histone H4 is a 103 amino acid protein, which belongs to the histone H4 family. Histone H4 localizes in the nucleus and is a core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Acetylation of histone H4 is necessary for chromatin decompaction during DNA replication.

实验方案

Product Specific Protocols
WB protocol for Acetyl-Histone H4 (Lys12) antibody 83095-1-RRDownload protocol
IHC protocol for Acetyl-Histone H4 (Lys12) antibody 83095-1-RRDownload protocol
IF protocol for Acetyl-Histone H4 (Lys12) antibody 83095-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols