验证数据展示
经过测试的应用
Positive WB detected in | Trichostatin A treated NIH/3T3 cells, HEK-293 cells, HSC-T6 cells, NIH/3T3 cells |
Positive IHC detected in | mouse small intestine tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | Caco-2 cells |
Positive Dot Blot detected in | peptide |
推荐稀释比
应用 | 推荐稀释比 |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:150-1:600 |
DOT BLOT | DOT BLOT : 1:10-1:100 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
83095-1-RR targets Acetyl-Histone H4 (Lys12) in WB, IHC, IF/ICC, Dot Blot, ELISA applications and shows reactivity with human, mouse, rat samples.
经测试应用 | WB, IHC, IF/ICC, Dot Blot, ELISA Application Description |
经测试反应性 | human, mouse, rat |
免疫原 | Peptide 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Recombinant |
产品类型 | Antibody |
全称 | histone cluster 1, H4a |
别名 | Acetyl Histone H4, Ac Histone H4-Lys12, Ac Histone H4 Lys12, Ac Histone H4 (Lys12), Ac Histone H4 |
观测分子量 | 12 kDa |
GenBank蛋白编号 | BC069654 |
基因名称 | HIST1H4A |
Gene ID (NCBI) | 8359 |
RRID | AB_3670809 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Protein A purification |
UNIPROT ID | P62805 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Histone H4 is a 103 amino acid protein, which belongs to the histone H4 family. Histone H4 localizes in the nucleus and is a core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Acetylation of histone H4 is necessary for chromatin decompaction during DNA replication.
实验方案
Product Specific Protocols | |
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WB protocol for Acetyl-Histone H4 (Lys12) antibody 83095-1-RR | Download protocol |
IHC protocol for Acetyl-Histone H4 (Lys12) antibody 83095-1-RR | Download protocol |
IF protocol for Acetyl-Histone H4 (Lys12) antibody 83095-1-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |