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验证数据展示

  • IF Staining of Hela using CL488-83171-4

    IF Staining of Hela using CL488-83171-4

    Immunofluorescent analysis of (4% PFA) fixed Hela cells using CoraLite® Plus 488 Acetyl-Histone H2B (Lys5) antibody (CL488-83171-4, Clone: 230539G10 ) at dilution of 1:200.

  • FC experiment of HeLa using CL488-83171-4

    FC experiment of HeLa using CL488-83171-4

    1x10^6 HeLa cells were intracellularly stained with 0.8 ug Coralite® Plus 488 Acetyl-Histone H2b (Lys5) Recombinant Antibody (CL488-83171-4, Clone:230539G10)(red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

CoraLite® Plus 488-conjugated Acetyl-Histone H2B (Lys5) Recombinant monoclonal antibody

Acetyl-Histone H2B (Lys5) Uni-rAb® Recombinant Antibody for IF/ICC, FC (Intra)
Cat No. CL488-83171-4

产品说明书

CloneNo. 230539G10

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

IF/ICC, FC (Intra)

别名

HIST2H2BE, Histone H2B, H2BC21, H2B/q, H2B.1

缓冲液配方:  PBS, Proclin 300, BSA, Glycerol
PBS, Proclin 300, BSA, Glycerol
偶联物:  CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive IF/ICC detected inHela cells
Positive FC (Intra) detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 83171-4-PBS

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-83171-4 targets Acetyl-Histone H2B (Lys5) in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human, mouse
免疫原

Peptide

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 histone cluster 2, H2be
别名 HIST2H2BE, Histone H2B, H2BC21, H2B/q, H2B.1
计算分子量 14 kDa
观测分子量15 kDa
GenBank蛋白编号BC005827
基因名称 Histone H2B
Gene ID (NCBI) 8349
RRIDAB_3673213
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ16778
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 Acetyl-Histone H2B (Lys5) antibody CL488-83171-4Download protocol
Standard Protocols
Click here to view our Standard Protocols
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