Chromatin was prepared from HeLa cells. Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 15 µg of cross-linked chromatin, 5 µg of Acetyl-Histone H2B (Lys5) (83171-4-RR) or 5 ug of Normal Rabbit IgG (98136-1-RR), and 20 µl of Protein A Magarose Beads. The immunoprecipitated DNA was quantified by real-time PCR. This data was developed using the same antibody clone with 83171-4-PBS in a different storage buffer formulation.

Trichostatin A treated and untreated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 83171-4-RR (Acetyl-Histone H2B (Lys5) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with Beta Actin Monoclonal antibody (66009-1-Ig) as loading control. This data was developed using the same antibody clone with 83171-4-PBS in a different storage buffer formulation.

Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Acetyl-Histone H2B (Lys5) antibody (83171-4-RR, Clone: 230539G10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83171-4-PBS in a different storage buffer formulation.

Immunofluorescent analysis of (4% PFA) fixed Trichostatin A treated HeLa cells using Acetyl-Histone H2B (Lys5) antibody (83171-4-RR, Clone: 230539G10 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83171-4-PBS in a different storage buffer formulation.