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验证数据展示

  • Dot Blot experiment using 83171-7-RR (same clone as 83171-7-PBS)

    Dot Blot experiment using 83171-7-RR (same clone as 83171-7-PBS)

    Dot blot analysis was used to confirm the specificity of 83171-7-RR Acetyl-Histone H2B (Lys15) antibody. peptides were spotted onto NC and probed with antibody at 1 µg/ml.The amount of peptide (μg/mL) spotted is indicated next to each row. This data was developed using the same antibody clone with 83171-7-PBS in a different storage buffer formulation.

  • ChIP experiment of HeLa using 83171-7-RR (same clone as 83171-7-PBS)

    ChIP experiment of HeLa using 83171-7-RR (same clone as 83171-7-PBS)

    Chromatin was prepared from HeLa cells. Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 15 µg of cross-linked chromatin, 5 µg of Acetyl-Histone H2B (Lys15) (83171-7-RR) or 5 ug of Normal Rabbit IgG (98136-1-RR), and 20 µl of Protein A Magarose Beads. The immunoprecipitated DNA was quantified by real-time PCR. This data was developed using the same antibody clone with 83171-7-PBS in a different storage buffer formulation.

  • WB analysis of NIH/3T3 using 83171-7-RR (same clone as 83171-7-PBS)

    WB analysis of NIH/3T3 using 83171-7-RR (same clone as 83171-7-PBS)

    Trichostatin A treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 83171-7-RR (Acetyl-Histone H2B (Lys15) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with Beta Actin Monoclonal antibody (66009-1-Ig) as loading control. This data was developed using the same antibody clone with 83171-7-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 83171-7-RR (same clone as 83171-7-PBS)

    IF Staining of HeLa using 83171-7-RR (same clone as 83171-7-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Trichostatin A treated HeLa cells using Acetyl-Histone H2B (Lys15) antibody (83171-7-RR, Clone: 250366G3 ) at dilution of 1:5000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83171-7-PBS in a different storage buffer formulation.

Acetyl-Histone H2B (Lys15) Recombinant monoclonal antibody, PBS Only

Acetyl-Histone H2B (Lys15) Uni-rAb® Recombinant Antibody for WB, IF/ICC, Dot Blot, Indirect ELISA, ChIP-qPCR
Cat No. 83171-7-PBS

产品说明书

CloneNo. 250366G3

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IF/ICC, Dot Blot, Indirect ELISA, ChIP-qPCR

别名

Ac Histone H2B, Ac Histone H2B (Lys15), Ac Histone H2B Lys15, Ac Histone H2B-Lys15, Acetyl Histone

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

83171-7-PBS targets Acetyl-Histone H2B (Lys15) in WB, IF/ICC, Dot Blot, Indirect ELISA, ChIP-qPCR applications and shows reactivity with human, mouse samples.

经测试应用 WB, IF/ICC, Dot Blot, Indirect ELISA, ChIP-qPCR Application Description
经测试反应性 human, mouse
免疫原

Peptide

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 histone cluster 2, H2be
别名 Ac Histone H2B, Ac Histone H2B (Lys15), Ac Histone H2B Lys15, Ac Histone H2B-Lys15, Acetyl Histone
计算分子量 14 kDa
观测分子量15 kDa
GenBank蛋白编号BC005827
基因名称 Histone H2B
Gene ID (NCBI) 8349
RRIDAB_3743465
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ16778
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Histones are small, highly basic proteins that consist of a globular domain with unstructured N- and C-terminal tails protruding from the main structure. Histone H3 is one of the five main histones that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. In addition to their role in DNA compartmentalization, histones also play crucial roles in various biologic processes, including gene expression and regulation, DNA repair, chromatin condensation, cell cycle progression, chromosome segregation, and apoptosis. The ability of histones to regulate chromatin dynamics primarily originates from various posttranslational modifications carried out by histone-modifying enzymes.

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