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验证数据展示

  • Dot Blot experiment using 83171-7-RR

    Dot Blot experiment using 83171-7-RR

    Dot blot analysis was used to confirm the specificity of 83171-7-RR Acetyl-Histone H2B (Lys15) antibody. peptides were spotted onto NC and probed with antibody at 1 µg/ml.The amount of peptide (μg/mL) spotted is indicated next to each row.

  • WB analysis of NIH/3T3 using 83171-7-RR

    WB analysis of NIH/3T3 using 83171-7-RR

    Trichostatin A treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 83171-7-RR (Acetyl-Histone H2B (Lys15) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with Beta Actin Monoclonal antibody (66009-1-Ig) as loading control.

Acetyl-Histone H2B (Lys15) Recombinant antibody

Acetyl-Histone H2B (Lys15) Uni-rAbTM Recombinant Antibody for WB, Dot Blot, ELISA
Cat No. 83171-7-RR

产品说明书

CloneNo. 250366G3

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, Dot Blot, ELISA

别名

Ac Histone H2B, Ac Histone H2B (Lys15), Ac Histone H2B Lys15, Ac Histone H2B-Lys15, Acetyl Histone

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inTrichostatin A treated NIH/3T3 cells
Positive Dot Blot detected inN/A

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
DOT BLOTDOT BLOT : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83171-7-RR targets Acetyl-Histone H2B (Lys15) in WB, Dot Blot, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, Dot Blot, ELISA Application Description
经测试反应性 human, mouse
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 histone cluster 2, H2be
别名 Ac Histone H2B, Ac Histone H2B (Lys15), Ac Histone H2B Lys15, Ac Histone H2B-Lys15, Acetyl Histone
计算分子量 14 kDa
观测分子量15 kDa
GenBank蛋白编号BC005827
基因名称 Histone H2B
Gene ID (NCBI) 8349
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ16778
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for Acetyl-Histone H2B (Lys15) antibody 83171-7-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols