验证数据展示
经过测试的应用
| Positive IF-P detected in | mouse kidney tissue |
Planning an IHC experiment? We recommend our IHCeasy® ATPBD3 Ready-To-Use IHC Kit. ATPBD3 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 84318-4-PBS
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-84318-4 targets ATPBD3 in IF-P applications and shows reactivity with human, mouse samples.
| 经测试应用 | IF-P Application Description |
| 经测试反应性 | human, mouse |
| 免疫原 |
CatNo: Ag35037 Product name: Recombinant human ATPBD3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 50-200 aa of NM_145232 Sequence: PGAVVAVGASGGKDSTVLAHVLRALAPRLGISLQLVAVDEGIGGYRDAALAAVRRQAARWELPLTVVAYEDLFGGWTMDAVARSTAGSGRSRSCCTFCGVLRRRALEEGARRVGATHIVTGHNADDMAETVLMNFLRGDAGRLARGGGLGS 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | ATP binding domain 3 |
| 别名 | CTU1, NCS6 |
| 计算分子量 | 36 kDa |
| 观测分子量 | 35 kDa |
| GenBank蛋白编号 | NM_145232 |
| 基因名称 | ATPBD3 |
| Gene ID (NCBI) | 90353 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q7Z7A3 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ATPBD3, also known as CTU1, NCS6, is a subunit of a highly conserved protein complex involved in the thiolation of wobble U34(PMID: 18391219). ATPBD3 directly binds tRNAs and probably acts by catalyzing the adenylation of tRNAs, an intermediate required for 2-thiolation.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 ATPBD3 antibody CL488-84318-4 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
