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验证数据展示

  • WB analysis using 84318-4-RR (same clone as 84318-4-PBS)

    WB analysis using 84318-4-RR (same clone as 84318-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84318-4-RR (ATPBD3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84318-4-PBS in a different storage buffer formulation.

  • IHC staining of mouse kidney using 84318-4-RR (same clone as 84318-4-PBS)

    IHC staining of mouse kidney using 84318-4-RR (same clone as 84318-4-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 84318-4-RR (ATPBD3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84318-4-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ATPBD3 antibody included
  • IHC staining of mouse kidney using 84318-4-RR (same clone as 84318-4-PBS)

    IHC staining of mouse kidney using 84318-4-RR (same clone as 84318-4-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 84318-4-RR (ATPBD3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84318-4-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ATPBD3 antibody included
  • IF Staining of mouse kidney using 84318-4-RR (same clone as 84318-4-PBS)

    IF Staining of mouse kidney using 84318-4-RR (same clone as 84318-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse kidney tissue using ATPBD3 antibody (84318-4-RR, Clone: 241630C4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84318-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84318-4-RR

    Affinity and Kinetic Characterization of 84318-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84318-4-RR against Human ATPBD3 were performed. The affinity constant is 0.406 nM.

ATPBD3 Recombinant monoclonal antibody, PBS Only

ATPBD3 Uni-rAb® Recombinant Antibody for WB, IHC, IF-P, Indirect ELISA
Cat No. 84318-4-PBS

产品说明书

CloneNo. 241630C4

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF-P, Indirect ELISA

别名

241630C4, CTU1, NCS6

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy® ATPBD3 Ready-To-Use IHC Kit. ATPBD3 primary antibody included.

产品信息

84318-4-PBS targets ATPBD3 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF-P, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag35037

Product name: Recombinant human ATPBD3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 50-200 aa of NM_145232

Sequence: PGAVVAVGASGGKDSTVLAHVLRALAPRLGISLQLVAVDEGIGGYRDAALAAVRRQAARWELPLTVVAYEDLFGGWTMDAVARSTAGSGRSRSCCTFCGVLRRRALEEGARRVGATHIVTGHNADDMAETVLMNFLRGDAGRLARGGGLGS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 ATP binding domain 3
别名 241630C4, CTU1, NCS6
计算分子量36 kDa
观测分子量 35 kDa
GenBank蛋白编号NM_145232
基因名称 ATPBD3
Gene ID (NCBI) 90353
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDQ7Z7A3
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ATPBD3, also known as CTU1, NCS6, is a subunit of a highly conserved protein complex involved in the thiolation of wobble U34(PMID: 18391219). ATPBD3 directly binds tRNAs and probably acts by catalyzing the adenylation of tRNAs, an intermediate required for 2-thiolation.

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