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验证数据展示

  • WB analysis using 84318-4-RR

    WB analysis using 84318-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84318-4-RR (ATPBD3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse kidney using 84318-4-RR

    IHC staining of mouse kidney using 84318-4-RR

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 84318-4-RR (ATPBD3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ATPBD3 antibody included
  • IHC staining of mouse kidney using 84318-4-RR

    IHC staining of mouse kidney using 84318-4-RR

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 84318-4-RR (ATPBD3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ATPBD3 antibody included
  • IF Staining of mouse kidney using 84318-4-RR

    IF Staining of mouse kidney using 84318-4-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse kidney tissue using ATPBD3 antibody (84318-4-RR, Clone: 241630C4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 84318-4-RR

    Affinity and Kinetic Characterization of 84318-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84318-4-RR against Human ATPBD3 were performed. The affinity constant is 0.406 nM.

ATPBD3 Recombinant monoclonal antibody

ATPBD3 Uni-rAb® Recombinant Antibody for WB, IHC, IF-P, ELISA
Cat No. 84318-4-RR

产品说明书

CloneNo. 241630C4

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF-P, ELISA

别名

241630C4, CTU1, NCS6

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inPC-3 cells, K-562 cells, HeLa cells, HEK-293 cells
Positive IHC detected inmouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse kidney tissue
Planning an IHC experiment? We recommend our IHCeasy® ATPBD3 Ready-To-Use IHC Kit. ATPBD3 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84318-4-RR targets ATPBD3 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF-P, ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag35037

Product name: Recombinant human ATPBD3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 50-200 aa of NM_145232

Sequence: PGAVVAVGASGGKDSTVLAHVLRALAPRLGISLQLVAVDEGIGGYRDAALAAVRRQAARWELPLTVVAYEDLFGGWTMDAVARSTAGSGRSRSCCTFCGVLRRRALEEGARRVGATHIVTGHNADDMAETVLMNFLRGDAGRLARGGGLGS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 ATP binding domain 3
别名 241630C4, CTU1, NCS6
计算分子量36 kDa
观测分子量 35 kDa
GenBank蛋白编号NM_145232
基因名称 ATPBD3
Gene ID (NCBI) 90353
RRIDAB_3671859
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDQ7Z7A3
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ATPBD3, also known as CTU1, NCS6, is a subunit of a highly conserved protein complex involved in the thiolation of wobble U34(PMID: 18391219). ATPBD3 directly binds tRNAs and probably acts by catalyzing the adenylation of tRNAs, an intermediate required for 2-thiolation.

实验方案

Product Specific Protocols
IF protocol for ATPBD3 antibody 84318-4-RRDownload protocol
IHC protocol for ATPBD3 antibody 84318-4-RRDownload protocol
WB protocol for ATPBD3 antibody 84318-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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