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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 66600-1-Ig (same clone as 66600-1-PBS)

    WB analysis using 66600-1-Ig (same clone as 66600-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66600-1-Ig (ATPB antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66600-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver using 66600-1-Ig (same clone as 66600-1-PBS)

    IHC staining of human liver using 66600-1-Ig (same clone as 66600-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66600-1-Ig (ATPB antibody) at dilution of 1:500 (under 10x lens). This data was developed using the same antibody clone with 66600-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ATP5B antibody included
  • IHC staining of human liver using 66600-1-Ig (same clone as 66600-1-PBS)

    IHC staining of human liver using 66600-1-Ig (same clone as 66600-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66600-1-Ig (ATPB antibody) at dilution of 1:500 (under 40x lens). This data was developed using the same antibody clone with 66600-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ATP5B antibody included
  • IF Staining of U2OS using 66600-1-Ig (same clone as 66600-1-PBS)

    IF Staining of U2OS using 66600-1-Ig (same clone as 66600-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using ATPB antibody (66600-1-Ig, Clone: 1A11B9 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-phalloidin (red). This data was developed using the same antibody clone with 66600-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 66600-1-Ig (same clone as 66600-1-PBS)

    IF Staining of HepG2 using 66600-1-Ig (same clone as 66600-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using ATPB antibody (66600-1-Ig, Clone: 1A11B9 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red). This data was developed using the same antibody clone with 66600-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 66600-1-Ig (same clone as 66600-1-PBS)

    IF Staining of HepG2 using 66600-1-Ig (same clone as 66600-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using ATPB antibody (66600-1-Ig, Clone: 1A11B9 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red). This data was developed using the same antibody clone with 66600-1-PBS in a different storage buffer formulation.

ATPB Monoclonal antibody, PBS Only

ATPB Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 66600-1-PBS

产品说明书

CloneNo. 1A11B9

宿主/亚型

Mouse / IgG2b

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

1A11B9, ATP synthase F1 subunit beta, ATP5B, ATP5F1B, ATPMB

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy ATP5B Ready-To-Use IHC Kit. ATP5B primary antibody included.

产品信息

66600-1-PBS targets ATPB in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag11177

Product name: Recombinant human ATPB protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 181-529 aa of BC016512

Sequence: EILVTGIKVVDLLAPYAKGGKIGLFGGAGVGKTVLIMELINNVAKAHGGYSVFAGVGERTREGNDLYHEMIESGVINLKDATSKVALVYGQMNEPPGARARVALTGLTVAEYFRDQEGQDVLLFIDNIFRFTQAGSEVSALLGRIPSAVGYQPTLATDMGTMQERITTTKKGSITSVQAIYVPADDLTDPAPATTFAHLDATTVLSRAIAELGIYPAVDPLDSTSRIMDPNIVGSEHYDVARGVQKILQDYKSLQDIIAILGMDELSEEDKLTVSRARKIQRFLSQPFQVAEVFTGHMGKLVPLKETIKGFQQILAGEYDHLPEQAFYMVGPIEEAVAKADKLAEEHSS

种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 ATP synthase, H+ transporting, mitochondrial F1 complex, beta polypeptide
别名 1A11B9, ATP synthase F1 subunit beta, ATP5B, ATP5F1B, ATPMB
计算分子量 529 aa, 57 kDa
观测分子量45-57 kDa
GenBank蛋白编号BC016512
基因名称 ATPB
Gene ID (NCBI) 506
RRIDAB_2881960
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP06576
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ATP5B, also named as ATPMB and ATPSB, belongs to the ATPase alpha/beta chains family. It is a high affinity HDL receptor for apolipoprotein A1. ATP5B is a subunit of mitochondrial ATP synthase (F1F0 ATP synthase or Complex V ). ATP5B has a calculated molecular mass of 57 kDa and ~5 kDa transit peptide can be removed in the mature form.