CoraLite® Plus 488-conjugated ATP5I Polyclonal antibody
ATP5I Polyclonal Antibody for IF/ICC
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
IF/ICC
Conjugate
CoraLite® Plus 488 Fluorescent Dye
验证数据展示
经过测试的应用
| Positive IF detected in | HeLa cells |
For other applications, we recommend the unconjugated version of this antibody, 16483-1-AP
推荐稀释比
| Application | Dilution |
|---|---|
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-16483 targets ATP5I in IF applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | IF/ICC |
| Tested Reactivity | human, mouse, rat |
| Immunogen | ATP5I fusion protein Ag9605 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | ATP synthase, H+ transporting, mitochondrial F0 complex, subunit E |
| Synonyms | ATP5I, ATP5K, ATPase subunit e |
| Calculated Molecular Weight | 69 aa, 8 kDa |
| Observed Molecular Weight | 8 kDa |
| GenBank Accession Number | BC003679 |
| Gene Symbol | ATP5I |
| Gene ID (NCBI) | 521 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P56385 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ATP5I(ATP synthase subunit e) is also named as ATP5K and belongs to the ATPase e subunit family. The ATP5I gene encodes the e subunit of the mitochondrial ATP synthase Fo complex. Mitochondrial membrane ATP synthase(F1F0 ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. Antisense ATP5I in a human HCC cell line inhibited cell growth suggesting that ATP5I acts through the MAP kinase pathway(PMID:11939412).
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 ATP5I antibody CL488-16483 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |