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验证数据展示

  • WB analysis using 84935-6-RR

    WB analysis using 84935-6-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84935-6-RR (ATG12 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon cancer using 84935-6-RR

    IHC staining of human colon cancer using 84935-6-RR

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 84935-6-RR (ATG12 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human colon cancer using 84935-6-RR

    IHC staining of human colon cancer using 84935-6-RR

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 84935-6-RR (ATG12 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 84935-6-RR

    Affinity and Kinetic Characterization of 84935-6-RR

    Biolayer interferometry (BLl) kinetic assays of 84935-6-RR against Human ATG12 were performed. The affinity constant is 0.637 nM.

ATG12 Recombinant monoclonal antibody

ATG12 Uni-rAb® Recombinant Antibody for WB, IHC, ELISA
Cat No. 84935-6-RR

产品说明书

CloneNo. 242483F1

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

242483F1, APG12, APG12 like, APG12L, APG12-like

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells, PC-3 cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84935-6-RR targets ATG12 in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag1791

Product name: Recombinant human ATG12 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-187 aa of BC012266

Sequence: MTSREHQVSLCNCVPLLRRLLCDAPWRKARPLHALSRYFRSRVSPSKMAEEPQSVLQLPTSIAAGGEGLTDVSPETTTPEPPSSAAVSPGTEEPAGDTKKKIDILLKAVGDTPIMKTKKWAVERTRTIQGLIDFIKKFLKLVASEQLFIYVNQSFAPSPDQEVGTLYECFGSDGKLVLHYCKSQAWG

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 ATG12 autophagy related 12 homolog (S. cerevisiae)
别名 242483F1, APG12, APG12 like, APG12L, APG12-like
计算分子量 15 kDa
观测分子量55 kDa
GenBank蛋白编号BC012266
基因名称 ATG12
Gene ID (NCBI) 9140
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDO94817
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ATG12, also named as APG12 and APG12L, belongs to the ATG12 family. It is required for autophagy. Free ATG12 is 15kd or 8kd. Conjugated to ATG5, ATG12-ATG5 show 48-55kd.

实验方案

Product Specific Protocols
IHC protocol for ATG12 antibody 84935-6-RRDownload protocol
WB protocol for ATG12 antibody 84935-6-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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