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验证数据展示

  • WB analysis using 84935-6-RR (same clone as 84935-6-PBS)

    WB analysis using 84935-6-RR (same clone as 84935-6-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84935-6-RR (ATG12 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84935-6-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 84935-6-RR (same clone as 84935-6-PBS)

    IHC staining of human colon cancer using 84935-6-RR (same clone as 84935-6-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 84935-6-RR (ATG12 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84935-6-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 84935-6-RR (same clone as 84935-6-PBS)

    IHC staining of human colon cancer using 84935-6-RR (same clone as 84935-6-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 84935-6-RR (ATG12 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84935-6-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84935-6-RR

    Affinity and Kinetic Characterization of 84935-6-RR

    Biolayer interferometry (BLl) kinetic assays of 84935-6-RR against Human ATG12 were performed. The affinity constant is 0.637 nM.

ATG12 Recombinant antibody, PBS Only

ATG12 Recombinant Antibody for WB, IHC, Indirect ELISA
Cat No. 84935-6-PBS

产品说明书

CloneNo. 242483F1

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, Indirect ELISA

别名

242483F1, APG12, APG12 like, APG12L, APG12-like

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

84935-6-PBS targets ATG12 in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human
免疫原 ATG12 fusion protein Ag1791 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 ATG12 autophagy related 12 homolog (S. cerevisiae)
别名 242483F1, APG12, APG12 like, APG12L, APG12-like
计算分子量 15 kDa
观测分子量55 kDa
GenBank蛋白编号BC012266
基因名称 ATG12
Gene ID (NCBI) 9140
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDO94817
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ATG12, also named as APG12 and APG12L, belongs to the ATG12 family. It is required for autophagy. Free ATG12 is 15kd or 8kd. Conjugated to ATG5, ATG12-ATG5 show 48-55kd.