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验证数据展示

  • WB analysis using 86086-1-RR

    WB analysis using 86086-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 86086-1-RR (ASCC2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis using 86086-1-RR

    WB analysis using 86086-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 86086-1-RR (ASCC2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon cancer using 86086-1-RR

    IHC staining of human colon cancer using 86086-1-RR

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 86086-1-RR (ASCC2 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HepG2 using 86086-1-RR

    IF Staining of HepG2 using 86086-1-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using ASCC2 antibody (86086-1-RR, Clone: 250566D5 ) at dilution of 1:300 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

ASCC2 Recombinant antibody

ASCC2 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 86086-1-RR

产品说明书

CloneNo. 250566D5

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

ASC-1 complex subunit p100, ASC1P100, RQT3

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells, mouse brain tissue, HepG2 cells, K-562 cells, MCF-7 cells, HEK-293T cells, rat brain tissue
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:150-1:600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

86086-1-RR targets ASCC2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 ASCC2 fusion protein Ag2100 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 activating signal cointegrator 1 complex subunit 2
别名 ASC-1 complex subunit p100, ASC1P100, RQT3
计算分子量 757 aa, 86 kDa
观测分子量 90-100 kDa
GenBank蛋白编号BC025368
基因名称 ASCC2
Gene ID (NCBI) 84164
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9H1I8
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ASCC2, also known as ASC1p100, is a subunit of the activating signal cointegrator 1 complex. ASCC2 plays a role in DNA damage repair as a component of the ASCC complex, recruiting ASCC3 and ALKBH3 to sites of DNA damage by binding to polyubiquitinated proteins that have 'Lys-63'-linked polyubiquitin chains. Additionally, it is part of the ASC-1 complex that enhances NF-kappa-B, SRF, and AP1 transactivation(PMID: 29144457, 12077347).

实验方案

Product Specific Protocols
WB protocol for ASCC2 antibody 86086-1-RRDownload protocol
IHC protocol for ASCC2 antibody 86086-1-RRDownload protocol
IF protocol for ASCC2 antibody 86086-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols