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验证数据展示

  • WB analysis using 86086-1-RR (same clone as 86086-1-PBS)

    WB analysis using 86086-1-RR (same clone as 86086-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86086-1-RR (ASCC2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86086-1-PBS in a different storage buffer formulation.

  • WB analysis using 86086-1-RR (same clone as 86086-1-PBS)

    WB analysis using 86086-1-RR (same clone as 86086-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86086-1-RR (ASCC2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86086-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 86086-1-RR (same clone as 86086-1-PBS)

    IHC staining of human colon cancer using 86086-1-RR (same clone as 86086-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 86086-1-RR (ASCC2 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86086-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 86086-1-RR (same clone as 86086-1-PBS)

    IF Staining of HepG2 using 86086-1-RR (same clone as 86086-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using ASCC2 antibody (86086-1-RR, Clone: 250566D5 ) at dilution of 1:300 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 86086-1-PBS in a different storage buffer formulation.

  • Cytometric bead array sample test of MP02784-1

    Cytometric bead array sample test of MP02784-1

    The mean ASCC2 concentration was determined to be 113.7 ng/mL in HEK-293T cell extract based on a 1.2 mg/mL extract load, 127.2 ng/mL in HeLa cell extract based on a 1.2 mg/mL extract load, 82.7 ng/mL in HepG2 cell extract based on a 1.2 mg/mL extract load.

  • Cytometric bead array standard curve of MP02784-1

    Cytometric bead array standard curve of MP02784-1

    Cytometric bead array standard curve of MP02784-1, ASCC2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 86086-1-PBS. Detection antibody: 86086-2-PBS. Standard: Ag2100. Range: 3.125-200 ng/mL.

  • Affinity and Kinetic Characterization of 86086-1-RR

    Affinity and Kinetic Characterization of 86086-1-RR

    Biolayer interferometry (BLl) kinetic assays of 86086-1-RR against Human ASCC2 were performed. The affinity constant is 26.5 pM.

ASCC2 Recombinant monoclonal antibody, PBS Only (Capture)

ASCC2 Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA
Cat No. 86086-1-PBS

产品说明书

CloneNo. 250566D5

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

别名

ASC-1 complex subunit p100, ASC1P100, RQT3

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

86086-1-PBS targets ASCC2 as part of a matched antibody pair:

MP02784-1: 86086-1-PBS capture and 86086-2-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag2100

Product name: Recombinant human ASCC2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 408-757 aa of BC025368

Sequence: RRKATDAKDPSVIEEPNGEPNGVTVTAEAVSQASSHPENSEEEECMGAAAAVGPAMCGVELDSLISQVKDLLPDLGEGFILACLEYYHYDPEQVINNILEERLAPTLSQLDRNLDREMKPDPTPLLTSRHNVFQNDEFDVFSRDSVDLSRVHKGKSTRKEENTRSLLNDKRAVAAQRQRYEQYSVVVEEVPLQPGESLPYHSVYYEDEYDDTYDGNQVGANDADSDDELISRRPFTIPQVLRTKVPREGQEEDDDDEEDDADEEAPKPDHFVQDPAVLREKAEARRMAFLAKKGYRHDSSTAVAGSPRGHGQSRETTQERRKKEANKATRANHNRRTMADRKRSKGMIPS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 activating signal cointegrator 1 complex subunit 2
别名 ASC-1 complex subunit p100, ASC1P100, RQT3
计算分子量 757 aa, 86 kDa
观测分子量 90-100 kDa
GenBank蛋白编号BC025368
基因名称 ASCC2
Gene ID (NCBI) 84164
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ9H1I8
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ASCC2, also known as ASC1p100, is a subunit of the activating signal cointegrator 1 complex. ASCC2 plays a role in DNA damage repair as a component of the ASCC complex, recruiting ASCC3 and ALKBH3 to sites of DNA damage by binding to polyubiquitinated proteins that have 'Lys-63'-linked polyubiquitin chains. Additionally, it is part of the ASC-1 complex that enhances NF-kappa-B, SRF, and AP1 transactivation(PMID: 29144457, 12077347).