验证数据展示
经过测试的应用
| Positive WB detected in | EC109 cells, HeLa cells, HEK-293 cells, HepG2 cells |
| Positive IHC detected in | human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:1500-1:6000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
66958-1-Ig targets ART1 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag28269 Product name: Recombinant human ART1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 26-289 aa of NM_004314 Sequence: MPITRRDLFSQEIQLDMALASFDDQYAGCAAAMTAALPDLNHTEFQANQVYADSWTLASSQWQERQARWPEWSLSPTRPSPPPLGFRDEHGVALLAYTANSPLHKEFNAAVREAGRSRAHYLHHFSFKTLHFLLTEALQLLGSGQRPPRCHQVFRGVHGLRFRPAGPRATVRLGGFASASLKHVAAQQFGEDTFFGIWTCLGAPIKGYSFFPGEEEVLIPPFETFQVINASRLAQGPARIYLRALGKHSTYNCEYIKDKKCKSGP 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | ADP-ribosyltransferase 1 |
| 别名 | ADP ribosyltransferase 1, ART2, CD296, Mono(ADP ribosyl)transferase 1, RT6 |
| 计算分子量 | 36 kDa |
| 观测分子量 | 34-36 kDa |
| GenBank蛋白编号 | NM_004314 |
| 基因名称 | ART1 |
| Gene ID (NCBI) | 417 |
| RRID | AB_2882281 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | P52961 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ART1 (ADP-ribosyltransferase 1) is an important mono-ADP-ribose transferase that can catalyze the mono-ADP-ribosylation of proteins including human neutrophil peptide-1 (HNP-1), fibroblast growth factor-2 (FGF-2) and platelet-derived growth factor-BB (PDGF-BB), and play a role in biological processes such as proliferation and invasion of cancer cells.
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for ART1 antibody 66958-1-Ig | Download protocol |
| WB protocol for ART1 antibody 66958-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Med Oncol Identification of the role of mono-ADP-ribosylation in colorectal cancer by integrated transcriptome analysis. | ||
BMC Cancer ART1 knockdown decreases the IL-6-induced proliferation of colorectal cancer cells
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