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验证数据展示

  • WB analysis using 66958-1-Ig

    WB analysis using 66958-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66958-1-Ig (ART1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • IHC staining of human kidney using 66958-1-Ig

    IHC staining of human kidney using 66958-1-Ig

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66958-1-Ig (ART1 antibody) at dilution of 1:3000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human kidney using 66958-1-Ig

    IHC staining of human kidney using 66958-1-Ig

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66958-1-Ig (ART1 antibody) at dilution of 1:3000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

ART1 Monoclonal antibody

ART1 Monoclonal Antibody for WB, IHC, ELISA
Cat No. 66958-1-Ig

产品说明书

CloneNo. 1D10A8
引用文献(2)

宿主/亚型

Mouse / IgG1

种属反应性

Human and More (2)

应用

WB, IHC, ELISA and More (1)

别名

ADP ribosyltransferase 1, ART1, ART2, CD296, Mono(ADP ribosyl)transferase 1, RT6

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inEC109 cells, HeLa cells, HEK-293 cells, HepG2 cells
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:1500-1:6000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66958-1-Ig targets ART1 in WB, IF, IHC, ELISA applications and shows reactivity with Human samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB, IF, IHC
经测试反应性 Human
文献引用反应性human, mouse
免疫原 ART1 fusion protein Ag28269 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 ADP-ribosyltransferase 1
别名 ADP ribosyltransferase 1, ART1, ART2, CD296, Mono(ADP ribosyl)transferase 1, RT6
计算分子量 36 kDa
观测分子量 34-36 kDa
GenBank蛋白编号NM_004314
基因名称 ART1
Gene ID (NCBI) 417
RRIDAB_2882281
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP52961
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ART1 (ADP-ribosyltransferase 1) is an important mono-ADP-ribose transferase which can catalyze the mono-ADP-ribosylation of proteins including human neutrophil peptide-1 (HNP-1), fibroblast growth factor-2 (FGF-2) and platelet-derived growth factor-BB (PDGF-BB), and play a role in biological processes such as proliferation and invasion of cancer cells.

实验方案

Product Specific Protocols
WB protocol for ART1 antibody 66958-1-IgDownload protocol
IHC protocol for ART1 antibody 66958-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Med Oncol

Identification of the role of mono-ADP-ribosylation in colorectal cancer by integrated transcriptome analysis.

Authors - Shuxian Zhang
View Article
human,mouseIHC,IF,WB

BMC Cancer

ART1 knockdown decreases the IL-6-induced proliferation of colorectal cancer cells

Authors - Ting Lin
  • KD Validated
View Article