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验证数据展示

  • WB analysis using 66958-1-Ig (same clone as 66958-1-PBS)

    WB analysis using 66958-1-Ig (same clone as 66958-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66958-1-Ig (ART1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66958-1-PBS in a different storage buffer formulation.

  • IHC staining of human kidney using 66958-1-Ig (same clone as 66958-1-PBS)

    IHC staining of human kidney using 66958-1-Ig (same clone as 66958-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66958-1-Ig (ART1 antibody) at dilution of 1:3000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66958-1-PBS in a different storage buffer formulation.

  • IHC staining of human kidney using 66958-1-Ig (same clone as 66958-1-PBS)

    IHC staining of human kidney using 66958-1-Ig (same clone as 66958-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66958-1-Ig (ART1 antibody) at dilution of 1:3000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66958-1-PBS in a different storage buffer formulation.

ART1 Monoclonal antibody, PBS Only

ART1 Monoclonal Antibody for WB, IHC, ELISA
Cat No. 66958-1-PBS

产品说明书

CloneNo. 1D10A8

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

WB, IHC, ELISA

别名

ADP ribosyltransferase 1, ART2, CD296, Mono(ADP ribosyl)transferase 1, RT6

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

66958-1-PBS targets ART1 in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag28269

Product name: Recombinant human ART1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 26-289 aa of NM_004314

Sequence: MPITRRDLFSQEIQLDMALASFDDQYAGCAAAMTAALPDLNHTEFQANQVYADSWTLASSQWQERQARWPEWSLSPTRPSPPPLGFRDEHGVALLAYTANSPLHKEFNAAVREAGRSRAHYLHHFSFKTLHFLLTEALQLLGSGQRPPRCHQVFRGVHGLRFRPAGPRATVRLGGFASASLKHVAAQQFGEDTFFGIWTCLGAPIKGYSFFPGEEEVLIPPFETFQVINASRLAQGPARIYLRALGKHSTYNCEYIKDKKCKSGP

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 ADP-ribosyltransferase 1
别名 ADP ribosyltransferase 1, ART2, CD296, Mono(ADP ribosyl)transferase 1, RT6
计算分子量 36 kDa
观测分子量 34-36 kDa
GenBank蛋白编号NM_004314
基因名称 ART1
Gene ID (NCBI) 417
RRIDAB_2882281
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDP52961
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ART1 (ADP-ribosyltransferase 1) is an important mono-ADP-ribose transferase which can catalyze the mono-ADP-ribosylation of proteins including human neutrophil peptide-1 (HNP-1), fibroblast growth factor-2 (FGF-2) and platelet-derived growth factor-BB (PDGF-BB), and play a role in biological processes such as proliferation and invasion of cancer cells.

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