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ACTRT3/ARPM1 Polyclonal antibody

ACTRT3/ARPM1 Polyclonal Antibody for IF/ICC, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

IF/ICC, IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 27580-1-AP

Print datasheet

Synonyms

actin related protein M1, ARPM1

验证数据展示

  • WB analysis using 27580-1-AP

    WB analysis using 27580-1-AP

    various lysates were subjected to SDS PAGE followed by western blot with 27580-1-AP (ARPM1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon using 27580-1-AP

    IHC staining of human colon using 27580-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 27580-1-AP (ARPM1 antibody) at dilution of 1:200 (under 10x lens).

  • IF Staining of U2OS using 27580-1-AP

    IF Staining of U2OS using 27580-1-AP

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using 27580-1-AP (ARPM1 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inrat testis tissue, mouse testis tissue
Positive IHC detected inhuman colon tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inU2OS cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

27580-1-AP targets ACTRT3/ARPM1 in WB, IF, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications IF/ICC, IHC, WB, ELISA
Cited ApplicationsWB
Tested Reactivity Human, mouse, rat
Cited Reactivitymouse
Immunogen ACTRT3/ARPM1 fusion protein Ag25951 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name actin related protein M1
Synonyms actin related protein M1, ARPM1
Calculated Molecular Weight 41 kDa
Observed Molecular Weight 41 kDa
GenBank Accession NumberBC007289
Gene Symbol ARPM1
Gene ID (NCBI) 84517
RRIDAB_2880914
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BYD9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for ACTRT3/ARPM1 antibody 27580-1-APDownload protocol
IHC protocol for ACTRT3/ARPM1 antibody 27580-1-APDownload protocol
IF protocol for ACTRT3/ARPM1 antibody 27580-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Front Cell Dev Biol

Loss of Profilin3 Impairs Spermiogenesis by Affecting Acrosome Biogenesis, Autophagy, Manchette Development and Mitochondrial Organization.

Authors - Naila Umer
View Article