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CoraLite® Plus 488-conjugated ARPC2 Monoclonal antibody

ARPC2 Monoclonal Antibody for IF/ICC,FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

IF/ICC,FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1D8E3

Cat No : CL488-68145

Print datasheet

Synonyms

ARC34, Arp2/3 complex 34 kDa subunit, ARPC2, p34 Arc, PNAS 139, PRO2446

验证数据展示

  • IF Staining of HeLa using CL488-68145

    IF Staining of HeLa using CL488-68145

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CoraLite® Plus 488 ARPC2 antibody (CL488-68145, Clone: 1D8E3 ) at dilution of 1:200.

  • FC experiment of HeLa using CL488-68145

    FC experiment of HeLa using CL488-68145

    1X10^6 HeLa cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human ARPC2 (CL488-68145, Clone:1D8E3) (red), or 0.8 ug CoraLite® Plus 488 Mouse IgG1 Isotype Control (MOPC-21) (CL488-65124, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive IF detected inHeLa cells
Positive FC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy ARPC2 Ready-To-Use IHC Kit. ARPC2 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 68145-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-68145 targets ARPC2 in IF, FC (Intra) applications and shows reactivity with Human, mouse, rat samples.

Tested Applications IF/ICC,FC (Intra)
Tested Reactivity Human, mouse, rat
Immunogen ARPC2 fusion protein Ag7082 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name actin related protein 2/3 complex, subunit 2, 34kDa
Synonyms ARC34, Arp2/3 complex 34 kDa subunit, ARPC2, p34 Arc, PNAS 139, PRO2446
Calculated Molecular Weight 34 kDa
Observed Molecular Weight34 kDa
GenBank Accession NumberBC000590
Gene Symbol ARPC2
Gene ID (NCBI) 10109
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDO15144
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 ARPC2 antibody CL488-68145Download protocol
FC protocol for CL Plus 488 ARPC2 antibody CL488-68145Download protocol
Standard Protocols
Click here to view our Standard Protocols