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ARPC2 Monoclonal antibody

ARPC2 Monoclonal Antibody for WB,IHC,IF/ICC,FC,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat, pig, rabbit

Applications

WB,IHC,IF/ICC,FC,ELISA

Conjugate

Unconjugated

CloneNo.

1D8E3

Cat No : 68145-1-Ig

Print datasheet

Synonyms

ARC34, Arp2/3 complex 34 kDa subunit, ARPC2, p34 Arc, PNAS 139, PRO2446

验证数据展示

  • WB analysis using 68145-1-Ig

    WB analysis using 68145-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68145-1-Ig (ARPC2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.

  • FC experiment of HeLa using 68145-1-Ig

    FC experiment of HeLa using 68145-1-Ig

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human ARPC2 (68145-1-Ig, Clone:1D8E3) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • IHC staining of human stomach cancer using 68145-1-Ig

    IHC staining of human stomach cancer using 68145-1-Ig

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 68145-1-Ig (ARPC2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ARPC2 antibody included
  • IHC staining of human stomach cancer using 68145-1-Ig

    IHC staining of human stomach cancer using 68145-1-Ig

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 68145-1-Ig (ARPC2 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ARPC2 antibody included
  • IF Staining of HeLa using 68145-1-Ig

    IF Staining of HeLa using 68145-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using ARPC2 antibody (68145-1-Ig, Clone: 1D8E3 ) at dilution of 1:2000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

经过测试的应用

Positive WB detected inHeLa cells, HEK-293 cells, HepG2 cells, K-562 cells, human peripheral blood platelets, pig brain tissue, rabbit brain tissue, rat brain tissue, mouse brain tissue
Positive IHC detected inhuman stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells
Positive FC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy ARPC2 Ready-To-Use IHC Kit. ARPC2 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)IF : 1:1000-1:4000
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68145-1-Ig targets ARPC2 in WB, IF, FC, IHC, ELISA applications and shows reactivity with Human, mouse, rat, pig, rabbit samples.

Tested Applications WB,IHC,IF/ICC,FC,ELISA
Tested Reactivity Human, mouse, rat, pig, rabbit
Immunogen ARPC2 fusion protein Ag7082 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name actin related protein 2/3 complex, subunit 2, 34kDa
Synonyms ARC34, Arp2/3 complex 34 kDa subunit, ARPC2, p34 Arc, PNAS 139, PRO2446
Calculated Molecular Weight 34 kDa
Observed Molecular Weight34 kDa
GenBank Accession NumberBC000590
Gene Symbol ARPC2
Gene ID (NCBI) 10109
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO15144
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for ARPC2 antibody 68145-1-IgDownload protocol
IHC protocol for ARPC2 antibody 68145-1-IgDownload protocol
IF protocol for ARPC2 antibody 68145-1-IgDownload protocol
FC protocol for ARPC2 antibody 68145-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols