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验证数据展示

  • WB analysis using 15006-1-AP (same clone as 15006-1-PBS)

    WB analysis using 15006-1-AP (same clone as 15006-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 15006-1-AP (ARIH2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15006-1-PBS in a different storage buffer formulation.

  • IP experiment of HeLa using 15006-1-AP (same clone as 15006-1-PBS)

    IP experiment of HeLa using 15006-1-AP (same clone as 15006-1-PBS)

    IP result of anti-ARIH2 (IP:15006-1-AP, 4ug; Detection:15006-1-AP 1:500) with HeLa cells lysate 1200ug. This data was developed using the same antibody clone with 15006-1-PBS in a different storage buffer formulation.

  • IF Staining of HEK-293 using 15006-1-AP (same clone as 15006-1-PBS)

    IF Staining of HEK-293 using 15006-1-AP (same clone as 15006-1-PBS)

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HEK-293 cells using 15006-1-AP (ARIH2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 15006-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

ARIH2 Polyclonal antibody, PBS Only

ARIH2 Polyclonal Antibody for WB, IF/ICC, IP, Indirect ELISA
Cat No. 15006-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, IP, Indirect ELISA

别名

ARI 2, ARI2, ARI-2, ARIH 2, E3 ubiquitin-protein ligase ARIH2

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

15006-1-PBS targets ARIH2 in WB, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag6507

Product name: Recombinant human ARIH2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 140-493 aa of BC000422

Sequence: AVCMQFVRKENLLSLACQHQFCRSCWEQHCSVLVKDGVGVGVSCMAQDCPLRTPEDFVFPLLPNEELREKYRRYLFRDYVESHYQLQLCPGADCPMVIRVQEPRARRVQCNRCNEVFCFKCRQMYHAPTDCATIRKWLTKCADDSETANYISAHTKDCPKCNICIEKNGGCNHMQCSKCKHDFCWMCLGDWKTHGSEYYECSRYKENPDIVNQSQQAQAREALKKYLFYFERWENHNKSLQLEAQTYQRIHEKIQERVMNNLGTWIDWQYLQNAAKLLAKCRYTLQYTYPYAYYMESGPRKKLFEYQQAQLEAEIENLSWKVERADSYDRGDLENQMHIAEQRRRTLLKDFHDT

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ariadne homolog 2 (Drosophila)
别名 ARI 2, ARI2, ARI-2, ARIH 2, E3 ubiquitin-protein ligase ARIH2
计算分子量 58 kDa
观测分子量 59 kDa
GenBank蛋白编号BC000422
基因名称 ARIH2
Gene ID (NCBI) 10425
RRIDAB_2290024
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDO95376
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ARIH2(Protein ariadne-2 homolog) is also named as ARI2, TRIAD1 and belongs to the RBR family and ariadne subfamily .It mediates 'Lys-48'-and 'Lys-63'-linked polyubiquitination and subsequent proteasomal degradation of modified proteins.

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