验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, HL-60 cells, mouse brain tissue, mouse kidney tissue |
| Positive IP detected in | HeLa cells |
| Positive IF/ICC detected in | HEK-293 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
15006-1-AP targets ARIH2 in WB, IHC, IF/ICC, IP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, IP, RIP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag6507 Product name: Recombinant human ARIH2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 140-493 aa of BC000422 Sequence: AVCMQFVRKENLLSLACQHQFCRSCWEQHCSVLVKDGVGVGVSCMAQDCPLRTPEDFVFPLLPNEELREKYRRYLFRDYVESHYQLQLCPGADCPMVIRVQEPRARRVQCNRCNEVFCFKCRQMYHAPTDCATIRKWLTKCADDSETANYISAHTKDCPKCNICIEKNGGCNHMQCSKCKHDFCWMCLGDWKTHGSEYYECSRYKENPDIVNQSQQAQAREALKKYLFYFERWENHNKSLQLEAQTYQRIHEKIQERVMNNLGTWIDWQYLQNAAKLLAKCRYTLQYTYPYAYYMESGPRKKLFEYQQAQLEAEIENLSWKVERADSYDRGDLENQMHIAEQRRRTLLKDFHDT 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | ariadne homolog 2 (Drosophila) |
| 别名 | ARI 2, ARI2, ARI-2, ARIH 2, E3 ubiquitin-protein ligase ARIH2 |
| 计算分子量 | 58 kDa |
| 观测分子量 | 59 kDa |
| GenBank蛋白编号 | BC000422 |
| 基因名称 | ARIH2 |
| Gene ID (NCBI) | 10425 |
| RRID | AB_2290024 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O95376 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ARIH2(Protein ariadne-2 homolog) is also named as ARI2, TRIAD1 and belongs to the RBR family and ariadne subfamily .It mediates 'Lys-48'-and 'Lys-63'-linked polyubiquitination and subsequent proteasomal degradation of modified proteins.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for ARIH2 antibody 15006-1-AP | Download protocol |
| IP protocol for ARIH2 antibody 15006-1-AP | Download protocol |
| WB protocol for ARIH2 antibody 15006-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cancer Cell Genome-wide CRISPR screens identify critical targets to enhance CAR-NK cell antitumor potency | ||
Nat Immunol ARIH2 is essential for embryogenesis, and its hematopoietic deficiency causes lethal activation of the immune system. | ||
Cell Death Dis ARIH2 regulates the proliferation, DNA damage and chemosensitivity of gastric cancer cells by reducing the stability of p21 via ubiquitination.
| ||
Oncogene PPDPF-mediated regulation of BCAA metabolism enhances mTORC1 activity and drives cholangiocarcinoma progression | ||
Oncogene The E3 ubiquitin ligase Triad1 influences development of Mll-Ell-induced acute myeloid leukemia. | ||
Int J Surg LINC00476 cooperates with ARIH2 and suppresses pancreatic cancer progression by inducing VIM ubiquitination.
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