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验证数据展示

  • IF Staining of HeLa using 82979-6-RR

    IF Staining of HeLa using 82979-6-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ARID1B antibody (82979-6-RR, Clone: 230222A11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1).CL594-Phalloidin (red).

  • FC experiment of SH-SY5Y using 82979-6-RR

    FC experiment of SH-SY5Y using 82979-6-RR

    1x10^6 SH-SY5Y cells were intracellularly stained with 0.25 ug ARID1B Recombinant antibody (82979-6-RR, Clone:230222A11) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Affinity and Kinetic Characterization of 82979-6-RR

    Affinity and Kinetic Characterization of 82979-6-RR

    Biolayer interferometry (BLl) kinetic assays of 82979-6-RR against Human ARID1B were performed. The affinity constant is 1.14 nM.

ARID1B Recombinant monoclonal antibody

ARID1B Recombinant Antibody for IF/ICC, FC (Intra), ELISA
Cat No. 82979-6-RR

产品说明书

CloneNo. 230222A11

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IF/ICC, FC (Intra), ELISA

别名

hOsa2, ELD/OSA1, DAN15, BRIGHT, BAF250b

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inSH-SY5Y cells
Planning an IHC experiment? We recommend our IHCeasy ARID1B Ready-To-Use IHC Kit. ARID1B primary antibody included.

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82979-6-RR targets ARID1B in IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag30643

Product name: Recombinant human ARID1B protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1370-1508 aa of NM_017519

Sequence: PAKRHEGDMYNMQYSSQQQEMYNQYGGSYSGPDRRPIQGQYPYPYSRERMQGPGQIQTHGIPPQMMGGPLQSSSSEGPQQNMWAARNDMPYPYQNRQGPGGPTQAPPYPGMNRTDDMMVPDQRINHESQWPSHVSQRQP

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 AT rich interactive domain 1B (SWI1-like)
别名 hOsa2, ELD/OSA1, DAN15, BRIGHT, BAF250b
计算分子量 236 kDa
GenBank蛋白编号NM_017519
基因名称 ARID1B
Gene ID (NCBI) 57492
RRIDAB_3670728
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ8NFD5
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for ARID1B antibody 82979-6-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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