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验证数据展示

  • IF Staining of HeLa using 82979-6-RR (same clone as 82979-6-PBS)

    IF Staining of HeLa using 82979-6-RR (same clone as 82979-6-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ARID1B antibody (82979-6-RR, Clone: 230222A11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1).CL594-Phalloidin (red). This data was developed using the same antibody clone with 82979-6-PBS in a different storage buffer formulation.

  • FC experiment of SH-SY5Y using 82979-6-RR (same clone as 82979-6-PBS)

    FC experiment of SH-SY5Y using 82979-6-RR (same clone as 82979-6-PBS)

    1x10^6 SH-SY5Y cells were intracellularly stained with 0.25 ug ARID1B Recombinant antibody (82979-6-RR, Clone:230222A11) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 82979-6-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82979-6-RR

    Affinity and Kinetic Characterization of 82979-6-RR

    Biolayer interferometry (BLl) kinetic assays of 82979-6-RR against Human ARID1B were performed. The affinity constant is 1.14 nM.

ARID1B Recombinant monoclonal antibody, PBS Only

ARID1B Uni-rAb® Recombinant Antibody for IF/ICC, FC (Intra), Indirect ELISA
Cat No. 82979-6-PBS

产品说明书

CloneNo. 230222A11

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IF/ICC, FC (Intra), Indirect ELISA

别名

hOsa2, ELD/OSA1, DAN15, BRIGHT, BAF250b

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy ARID1B Ready-To-Use IHC Kit. ARID1B primary antibody included.

产品信息

82979-6-PBS targets ARID1B in IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

经测试应用 IF/ICC, FC (Intra), Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag30643

Product name: Recombinant human ARID1B protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1370-1508 aa of NM_017519

Sequence: PAKRHEGDMYNMQYSSQQQEMYNQYGGSYSGPDRRPIQGQYPYPYSRERMQGPGQIQTHGIPPQMMGGPLQSSSSEGPQQNMWAARNDMPYPYQNRQGPGGPTQAPPYPGMNRTDDMMVPDQRINHESQWPSHVSQRQP

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 AT rich interactive domain 1B (SWI1-like)
别名 hOsa2, ELD/OSA1, DAN15, BRIGHT, BAF250b
计算分子量 236 kDa
GenBank蛋白编号NM_017519
基因名称 ARID1B
Gene ID (NCBI) 57492
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ8NFD5
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C
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