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CoraLite® Plus 488-conjugated ARHGEF16 Monoclonal antibody

ARHGEF16 Monoclonal Antibody for FC (Intra), IF/ICC

Host / Isotype

Mouse / IgG2b

Reactivity

Human

Applications

FC (Intra), IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1D8E3

Cat No : CL488-67975

Print datasheet

Synonyms

ARHGEF16, GEF16, NBR

验证数据展示

  • IF Staining of MCF-7 using CL488-67975

    IF Staining of MCF-7 using CL488-67975

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using CoraLite® Plus 488 ARHGEF16 antibody (CL488-67975, Clone: 1D8E3 ) at dilution of 1:200.

  • FC experiment of MCF-7 using CL488-67975

    FC experiment of MCF-7 using CL488-67975

    1X10^6 MCF-7 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human ARHGEF16 (CL488-67975, Clone:1D8E3) (red), or 0.4 ug Mouse IgG2b Isotype Control (CL488-66360-3, Clone: K11B8C4B5) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive IF detected inMCF-7 cells
Positive FC detected inMCF-7 cells
For other applications, we recommend the unconjugated version of this antibody, 67975-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67975 targets ARHGEF16 in IF, FC (Intra) applications and shows reactivity with Human samples.

Tested Applications FC (Intra), IF/ICC
Tested Reactivity Human
Immunogen ARHGEF16 fusion protein Ag28675 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name Rho guanine exchange factor (GEF) 16
Synonyms ARHGEF16, GEF16, NBR
Calculated Molecular Weight 80 kDa
Observed Molecular Weight80 kDa
GenBank Accession NumberBC002681
Gene Symbol ARHGEF16
Gene ID (NCBI) 27237
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ5VV41
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Small GTPases of the Rho family are critical regulators of various cellular functions including actin cytoskeleton organization, activation of kinase cascades, mitogenesis, transcriptional activation and stimulation of DNA synthesis. Rho proteins cycle between a biologically active GTP-bound state and an inactive GDP-bound state. Rho guanine nucleotide exchange factor (GEF) has a crucial role in activating small GTPase by exchange GDP for GTP. ARGEF16 is one of the multiple GEF members. Although the specific function of this protein is not known yet, it is thought to be involved in protein-protein and protein-lipid interactions.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 ARHGEF16 antibody CL488-67975Download protocol
FC protocol for CL Plus 488 ARHGEF16 antibody CL488-67975Download protocol
Standard Protocols
Click here to view our Standard Protocols