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验证数据展示

  • WB analysis using 84149-4-RR (same clone as 84149-4-PBS)

    WB analysis using 84149-4-RR (same clone as 84149-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84149-4-RR (ARHGEF11 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84149-4-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP01063-3

    Cytometric bead array standard curve of MP01063-3

    Cytometric bead array standard curve of MP01063-3, ARHGEF11 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84149-4-PBS. Detection antibody: 84149-2-PBS. Standard: Ag33104. Range: 0.313-40 ng/mL.

  • Affinity and Kinetic Characterization of 84149-4-PBS

    Affinity and Kinetic Characterization of 84149-4-PBS

    Biolayer interferometry (BLl) kinetic assay of 84149-4-PBS against Human ARHGEF11 was performed. The affinity constant is 0.707 nM.

  • Affinity and Kinetic Characterization of 84149-4-RR

    Affinity and Kinetic Characterization of 84149-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84149-4-RR against Human ARHGEF11 were performed. The affinity constant is 0.742 nM.

ARHGEF11 Recombinant antibody, PBS Only (Capture)

ARHGEF11 Uni-rAbTM Recombinant Antibody for WB, Cytometric bead array, Indirect ELISA
Cat No. 84149-4-PBS

产品说明书

CloneNo. 241427G2

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, Cytometric bead array, Indirect ELISA

别名

PDZ RHOGEF, KIAA0380, GTRAP48, 241427G2

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

84149-4-PBS targets ARHGEF11 as part of a matched antibody pair:

MP01063-3: 84149-4-PBS capture and 84149-2-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human
免疫原 ARHGEF11 fusion protein Ag33104 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 Rho guanine nucleotide exchange factor (GEF) 11
别名 PDZ RHOGEF, KIAA0380, GTRAP48, 241427G2
计算分子量168 kDa
观测分子量220 kDa
基因名称 ARHGEF11
Gene ID (NCBI) 9826
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDO15085
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ARHGEF11 (Rho guanine nucleotide exchange factor 11) is a specific guanine nucleotide exchange factor (GEF) for RhoA, which is a critical regulator of actin cytoskeleton dynamics and organization of dendritic spines and inhibitor of spine maintenance (PMID: 28036092). ARHGEF11 has been proven to promote tumor metastasis in glioblastoma and ovarian carcinoma (PMID: 33122451).