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验证数据展示

  • WB analysis using 84149-4-RR

    WB analysis using 84149-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84149-4-RR (ARHGEF11 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 84149-4-RR

    Affinity and Kinetic Characterization of 84149-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84149-4-RR against Human ARHGEF11 were performed. The affinity constant is 0.742 nM.

ARHGEF11 Recombinant monoclonal antibody

ARHGEF11 Uni-rAb® Recombinant Antibody for WB, ELISA
Cat No. 84149-4-RR

产品说明书

CloneNo. 241427G2

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, ELISA

别名

PDZ RHOGEF, KIAA0380, GTRAP48, 241427G2

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHEK-293T cells, HuH-7 cells, COLO 205 cells, MDA-MB-453 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:16000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84149-4-RR targets ARHGEF11 in WB, ELISA applications and shows reactivity with human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag33104

Product name: Recombinant human ARHGEF11 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1223-1522 aa of

Sequence: ETQAAQEPEDDLTPTPSVISVTSHPWDPGSPGQAPPGGEGDNTQLAGLEGERPEQEDMGLCSLEHLPPRTRNSGIWESPELDRNLAEDASSTEAAGGYKVVRKAEVAGSKVVPALPESGQSEPGPPEVEGGTKATGNCFYVSMPSGPPDSSTDHSEAPMSPPQPDSLPAGQTEPQPQLQGGNDDPRRPSRSPPSLALRDVGMIFHTIEQLTLKLNRLKDMELAHRELLKSLGGESSGGTTPVGSFHTEAARWTDGSLSPPAKEPLASDSRNSHELGPCPEDGSDAPLEDSTADAAASPGP

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 Rho guanine nucleotide exchange factor (GEF) 11
别名 PDZ RHOGEF, KIAA0380, GTRAP48, 241427G2
计算分子量168 kDa
观测分子量220 kDa
基因名称 ARHGEF11
Gene ID (NCBI) 9826
RRIDAB_3671714
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDO15085
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ARHGEF11 (Rho guanine nucleotide exchange factor 11) is a specific guanine nucleotide exchange factor (GEF) for RhoA, which is a critical regulator of actin cytoskeleton dynamics and organization of dendritic spines and inhibitor of spine maintenance (PMID: 28036092). ARHGEF11 has been proven to promote tumor metastasis in glioblastoma and ovarian carcinoma (PMID: 33122451).

实验方案

Product Specific Protocols
WB protocol for ARHGEF11 antibody 84149-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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