• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • FC experiment of HepG2 using CL488-22183

    FC experiment of HepG2 using CL488-22183

    1x10^6 HepG2 cells were intracellularly stained with 0.8 ug Coralite® Plus 488 Arhgap21 Polyclonal Antibody (CL488-22183)(red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Featured Product
  • KD/KO验证

CoraLite® Plus 488-conjugated ARHGAP21 Polyclonal antibody

ARHGAP21 Polyclonal Antibody for FC (Intra)
Cat No. CL488-22183

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, rat

应用

FC (Intra)

别名

Rho-type GTPase-activating protein 21, Rho GTPase-activating protein 10, KIAA1424, ARHGAP10

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive FC (Intra) detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy ARHGAP21 Ready-To-Use IHC Kit. ARHGAP21 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 22183-1-AP

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-22183 targets ARHGAP21 in FC (Intra) applications and shows reactivity with human, rat samples.

经测试应用 FC (Intra) Application Description
经测试反应性 human, rat
免疫原 ARHGAP21 fusion protein Ag16515 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 Rho GTPase activating protein 21
别名 Rho-type GTPase-activating protein 21, Rho GTPase-activating protein 10, KIAA1424, ARHGAP10
计算分子量 1957 aa, 217 kDa
观测分子量 250 kDa
GenBank蛋白编号BC118915
基因名称 ARHGAP21
Gene ID (NCBI) 57584
RRIDAB_3672740
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ5T5U3
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 ARHGAP21 antibody CL488-22183Download protocol
Standard Protocols
Click here to view our Standard Protocols