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ARHGAP21 Polyclonal antibody

ARHGAP21 Polyclonal Antibody for IF/ICC, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

IF/ICC, IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 22183-1-AP

Print datasheet

Synonyms

ARHGAP21, KIAA1424

验证数据展示

  • WB analysis using 22183-1-AP

    WB analysis using 22183-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 22183-1-AP (ARHGAP21 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of human ovary using 22183-1-AP

    IHC staining of human ovary using 22183-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tissue slide using 22183-1-AP (ARHGAP21 Antibody) at dilution of 1:50 (under 40x lens).

  • IF Staining of HepG2 using 22183-1-AP

    IF Staining of HepG2 using 22183-1-AP

    Immunofluorescent analysis of HepG2 cells using 22183-1-AP (ARHGAP21 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inHeLa cells, PC-3 cells
Positive IHC detected inhuman ovary tissue, human prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:200
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 22183-1-AP is ARHGAP21 Fusion Protein expressed in E. coli.

Tested Applications IF/ICC, IHC, WB, ELISA
Cited ApplicationsWB, IF
Tested Reactivity human, rat
Cited Reactivityhuman
Immunogen ARHGAP21 fusion protein Ag16515 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name Rho GTPase activating protein 21
Synonyms ARHGAP21, KIAA1424
Calculated Molecular Weight 1957 aa, 217 kDa
Observed Molecular Weight 250 kDa
GenBank Accession NumberBC118915
Gene Symbol ARHGAP21
Gene ID (NCBI) 57584
RRIDAB_2879018
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ5T5U3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for ARHGAP21 antibody 22183-1-APDownload protocol
IHC protocol for ARHGAP21 antibody 22183-1-APDownload protocol
IF protocol for ARHGAP21 antibody 22183-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Sci Adv

Phosphorylation-dependent recognition of diverse protein targets by the cryptic GK domain of MAGI MAGUKs

Authors - Meng Zhang
  • KD Validated
View Article
humanWB,IF

Elife

PTEN controls glandular morphogenesis through a juxtamembrane β-Arrestin1/ARHGAP21 scaffolding complex.

Authors - Arman Javadi
View Article