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  • Featured Product
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ARF4 Monoclonal antibody

ARF4 Monoclonal Antibody for FC, IF/ICC, WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, mouse, rat

Applications

FC, IF/ICC, WB, ELISA

Conjugate

Unconjugated

CloneNo.

2A4B8

Cat No : 68204-1-Ig

Print datasheet

Synonyms

ADP ribosylation factor 4, ARF2, ARF4

验证数据展示

  • WB analysis using 68204-1-Ig

    WB analysis using 68204-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68204-1-Ig (ARF4 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • WB analysis of A549 using 68204-1-Ig

    WB analysis of A549 using 68204-1-Ig

    A549 cells were subjected to SDS PAGE followed by western blot with 68204-1-Ig (ARF4 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • WB analysis using 68204-1-Ig

    WB analysis using 68204-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68204-1-Ig (ARF4 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 68204-1-Ig

    WB analysis of HeLa using 68204-1-Ig

    WB result of ARF4 antibody (68204-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ARF4 transfected HeLa cells.

  • WB analysis using 68204-1-Ig

    WB analysis using 68204-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68204-1-Ig (ARF4 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 68204-1-Ig

    IF Staining of HepG2 using 68204-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using ARF4 antibody (68204-1-Ig, Clone: 2A4B8 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • FC experiment of K-562 using 68204-1-Ig

    FC experiment of K-562 using 68204-1-Ig

    1X10^6 K-562 cells were intracellularly stained with 0.4 ug Anti-Human ARF4 (68204-1-Ig, Clone:2A4B8) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2b Isotype Control (66360-3-Ig, Clone: K11B8C4B5) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive WB detected inLNCaP cells, HEK-293 cells, rat brain tissue, A549 cells, mouse brain tissue, HeLa cells, HepG2 cells, Jurkat cells
Positive IF detected inHepG2 cells
Positive FC detected inK-562 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)IF : 1:400-1:1600
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68204-1-Ig targets ARF4 in WB, IF, FC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications FC, IF/ICC, WB, ELISA
Tested Reactivity Human, mouse, rat
Immunogen ARF4 fusion protein Ag25522 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name ADP-ribosylation factor 4
Synonyms ADP ribosylation factor 4, ARF2, ARF4
Calculated Molecular Weight 180 aa, 21 kDa
Observed Molecular Weight18 kDa
GenBank Accession NumberBC003364
Gene Symbol ARF4
Gene ID (NCBI) 378
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP18085
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ARF4, also named as ARF2, belongs to the small GTPase superfamily and Arf family. ADP-ribosylation factors (ARFs) are members of the ARF family of GTP-binding proteins of the Ras superfamily, with 20 kDa protein size. ARFs bind and regulate GTP/GDP cycle by alternating between the active GTP-bound and inactive GDP-bound conformations. ARF family proteins are essential and ubiquitous in eukaryotes. Six highly conserved members of the family have been identified in mammalian cells. They function in vesicular traffic and actin remodelling and other bioprocesses in cells. ARF4 and ARF5 are class II ARFs. Their function are not fully described. (PMID: 7759471, PMID: 16042562). This antibody can bind ARFs for the close sequences.

实验方案

Product Specific Protocols
WB protocol for ARF4 antibody 68204-1-IgDownload protocol
IF protocol for ARF4 antibody 68204-1-IgDownload protocol
FC protocol for ARF4 antibody 68204-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols