验证数据展示
经过测试的应用
| Positive IF-P detected in | mouse brain tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL594-67077 targets APBB1 in IF-P applications and shows reactivity with Human, Mouse, Rat, Pig samples.
| 经测试应用 | IF-P Application Description |
| 经测试反应性 | Human, Mouse, Rat, Pig |
| 免疫原 | APBB1 fusion protein Ag27436 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | amyloid beta (A4) precursor protein-binding, family B, member 1 (Fe65) |
| 别名 | APBB1, FE65, Protein Fe65, RIR |
| 计算分子量 | 708 aa, 77 kDa |
| GenBank蛋白编号 | BC010854 |
| 基因名称 | APBB1 |
| Gene ID (NCBI) | 322 |
| RRID | AB_2920084 |
| 偶联类型 | CoraLite®594 Fluorescent Dye |
| 最大激发/发射波长 | 588 nm / 604 nm |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | O00213 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
APBB1(Amyloid-beta A4 precursor protein-binding family B member 1) encoded FE65 protein. It was known as a binding partner of APP in the Alzheimer's disease studies, and expressed at high levels in brain especially in cerebellum, hippocampus, and cortex. FE65 and FE65-like (FE65L or FE65L1) proteins are cytoplasmic adaptor proteins that possess two phosphotyrosine binding domains (PTB1 and PTB2) and one WW binding domain (PMID:22429478). After phosphorylation modification, the band of FE65 protein would appear around 100 kDa. However, some tested a non-specific band at 55-60 kDa, it was refer to as FE65-like protein(PMID:12843239).
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL594 APBB1 antibody CL594-67077 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |