验证数据展示
经过测试的应用
| Positive IF/ICC detected in | HEK-293 cells |
For other applications, we recommend the unconjugated version of this antibody, 66533-1-Ig
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL594-66533 targets AP5Z1/SPG48 in IF/ICC applications and shows reactivity with Human, rat samples.
| 经测试应用 | IF/ICC Application Description |
| 经测试反应性 | Human, rat |
| 免疫原 | AP5Z1/SPG48 fusion protein Ag26961 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | KIAA0415 |
| 别名 | KIAA0415, SPG48 |
| 计算分子量 | 807 aa, 88 kDa |
| 观测分子量 | 88 kDa, 160 kDa |
| GenBank蛋白编号 | BC037399 |
| 基因名称 | AP5Z1/SPG48 |
| Gene ID (NCBI) | 9907 |
| RRID | AB_2920003 |
| 偶联类型 | CoraLite®594 Fluorescent Dye |
| 最大激发/发射波长 | 588 nm / 604 nm |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | O43299 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
AP5Z1, also known as SPG48 and KIAA0415, is a part of AP-5. Biallelic mutations in the AP5Z1 gene encoding the AP5Z1 subunit have been described in a small number of patients with hereditary spastic paraplegia (HSP). AP5Z1 physically interacts with other HSP proteins and that patient cells are sensitive to DNA damaging drugs.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL594 AP5Z1/SPG48 antibody CL594-66533 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |