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验证数据展示

  • WB analysis using 66533-1-Ig

    WB analysis using 66533-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66533-1-Ig (Zeta5 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IF Staining of HEK-293 using 66533-1-Ig

    IF Staining of HEK-293 using 66533-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using AP5Z1/SPG48 antibody (66533-1-Ig, Clone: 1G1D7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).

  • IHC staining of human liver cancer using 66533-1-Ig

    IHC staining of human liver cancer using 66533-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66533-1-Ig (AP5Z1/SPG48 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

AP5Z1/SPG48 Monoclonal antibody

AP5Z1/SPG48 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 66533-1-Ig

产品说明书

CloneNo. 1G1D7

宿主/亚型

Mouse / IgG1

种属反应性

Human, rat

应用

WB, IHC, IF/ICC, ELISA

别名

AP-5 complex subunit zeta-1, AP5Z1, AP5Z1/SPG48, KIAA0415, SPG48, Zeta5

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inJurkat cells, HL-60 cells, Caco-2 cells, COLO 320 cells, HSC-T6 cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHEK-293 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:5000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66533-1-Ig targets AP5Z1/SPG48 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 Human, rat
免疫原 AP5Z1/SPG48 fusion protein Ag26961 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 KIAA0415
别名 AP-5 complex subunit zeta-1, AP5Z1, AP5Z1/SPG48, KIAA0415, SPG48, Zeta5
计算分子量 807 aa, 88 kDa
观测分子量 88 kDa
GenBank蛋白编号BC037399
基因名称 AP5Z1/SPG48
Gene ID (NCBI) 9907
RRIDAB_2881896
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDO43299
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

AP5Z1, also known as SPG48 and KIAA0415, is a part of AP-5. Biallelic mutations in the AP5Z1 gene encoding the AP5Z1 subunit have been described in a small number of patients with hereditary spastic paraplegia (HSP). AP5Z1 physically interacts with other HSP proteins and that patient cells are sensitive to DNA damaging drugs.

实验方案

Product Specific Protocols
WB protocol for AP5Z1/SPG48 antibody 66533-1-IgDownload protocol
IHC protocol for AP5Z1/SPG48 antibody 66533-1-IgDownload protocol
IF protocol for AP5Z1/SPG48 antibody 66533-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols