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AOC3 Monoclonal antibody

AOC3 Monoclonal Antibody for IF-P, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human and More (1)

Applications

IF-P, IHC, WB, ELISA

Conjugate

Unconjugated

CloneNo.

2A6G3

Cat No : 66834-1-Ig

Print datasheet

Synonyms

AOC3, Copper amine oxidase, HPAO, Membrane primary amine oxidase, SSAO, VAP 1, VAP1, Vascular adhesion protein 1

验证数据展示

  • WB analysis of human ileum using 66834-1-Ig

    WB analysis of human ileum using 66834-1-Ig

    human ileum tissue were subjected to SDS PAGE followed by western blot with 66834-1-Ig (AOC3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver cancer using 66834-1-Ig

    IHC staining of human liver cancer using 66834-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66834-1-Ig (AOC3 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human liver cancer using 66834-1-Ig

    IHC staining of human liver cancer using 66834-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66834-1-Ig (AOC3 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of human liver cancer using 66834-1-Ig

    IF Staining of human liver cancer using 66834-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using AOC3 antibody (66834-1-Ig, Clone: 2A6G3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • IF Staining of human liver cancer using 66834-1-Ig

    IF Staining of human liver cancer using 66834-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using AOC3 antibody (66834-1-Ig, Clone: 2A6G3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

经过测试的应用

Positive WB detected inhuman ileum tissue
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhuman liver cancer tissue
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:3000-1:8000
Immunohistochemistry (IHC)IHC : 1:500-1:1200
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IHCSee 1 publications below

产品信息

66834-1-Ig targets AOC3 in WB, IF, IHC, ELISA applications and shows reactivity with Human samples.

Tested Applications IF-P, IHC, WB, ELISA
Cited ApplicationsIHC
Tested Reactivity Human
Cited Reactivityhuman
Immunogen AOC3 fusion protein Ag5908 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name amine oxidase, copper containing 3 (vascular adhesion protein 1)
Synonyms AOC3, Copper amine oxidase, HPAO, Membrane primary amine oxidase, SSAO, VAP 1, VAP1, Vascular adhesion protein 1
Calculated Molecular Weight 85 kDa
Observed Molecular Weight 85-90 kDa
GenBank Accession NumberBC050549
Gene Symbol AOC3
Gene ID (NCBI) 8639
RRIDAB_2882177
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ16853
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

AOC3(membrane primary amine oxidase) is also name as copper amine oxidase, HPAO, SSAO, VAP1 and belongs to the copper/topaquinone oxidase family. It is expressed on the surface of endothelial cells and is involved in leukocyte trafficking between blood and tissues under physiologic and pathologic conditions (PMID:17947691). AOC3 is highly expressed in adipocytes and smooth muscle cells and it can be N- and O-glycosylated (PMID:16046623). AOC3 has molecular mass of 70-90kD, and can exsit as a homodimer(165-185kD) and trimer(240~260kD) (PMID:10595925, 8625974).

实验方案

Product Specific Protocols
WB protocol for AOC3 antibody 66834-1-IgDownload protocol
IHC protocol for AOC3 antibody 66834-1-IgDownload protocol
IF protocol for AOC3 antibody 66834-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC

Front Immunol

A novel immune cell signature for predicting osteosarcoma prognosis and guiding therapy

Authors - Runsang Pan
View Article