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验证数据展示

  • WB analysis of human ileum using 66834-1-Ig (same clone as 66834-1-PBS)

    WB analysis of human ileum using 66834-1-Ig (same clone as 66834-1-PBS)

    human ileum tissue were subjected to SDS PAGE followed by western blot with 66834-1-Ig (AOC3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66834-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 66834-1-Ig (same clone as 66834-1-PBS)

    IHC staining of human liver cancer using 66834-1-Ig (same clone as 66834-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66834-1-Ig (AOC3 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66834-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 66834-1-Ig (same clone as 66834-1-PBS)

    IHC staining of human liver cancer using 66834-1-Ig (same clone as 66834-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66834-1-Ig (AOC3 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66834-1-PBS in a different storage buffer formulation.

  • IF Staining of human liver cancer using 66834-1-Ig (same clone as 66834-1-PBS)

    IF Staining of human liver cancer using 66834-1-Ig (same clone as 66834-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using AOC3 antibody (66834-1-Ig, Clone: 2A6G3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66834-1-PBS in a different storage buffer formulation.

  • IF Staining of human liver cancer using 66834-1-Ig (same clone as 66834-1-PBS)

    IF Staining of human liver cancer using 66834-1-Ig (same clone as 66834-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using AOC3 antibody (66834-1-Ig, Clone: 2A6G3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66834-1-PBS in a different storage buffer formulation.

VAP1/AOC3 Monoclonal antibody, PBS Only

VAP1/AOC3 Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA
Cat No. 66834-1-PBS

产品说明书

CloneNo. 2A6G3

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

WB, IHC, IF-P, Indirect ELISA

别名

AOC3, VAP1, 2A6G3, Amine oxidase [copper-containing] 3, Amine oxidase copper-containing 3

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

66834-1-PBS targets VAP1/AOC3 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF-P, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag5908

Product name: Recombinant human AOC3 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 408-763 aa of BC050549

Sequence: ATYVDWHFLLESQAPKTIRDAFCVFEQNQGLPLRRHHSDLYSHYFGGLAETVLVVRSMSTLLNYDYVWDTVFHPSGAIEIRFYATGYISSAFLFGATGKYGNQVSEHTLGTVHTHSAHFKVDLDVAGLENWVWAEDMVFVPMAVPWSPEHQLQRLQVTRKLLEMEEQAAFLVGSATPRYLYLASNHSNKWGHPRGYRIQMLSFAGEPLPQNSSMARGFSWERYQLAVTQRKEEEPSSSSVFNQNDPWAPTVDFSDFINNETIAGKDLVAWVTAGFLHIPHAEDIPNTVTVGNGVGFFLRPYNFFDEDPSFYSADSIYFRGDQDAGACEVNPLACLPQAAACAPDLPAFSHGGFSHN

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 amine oxidase, copper containing 3 (vascular adhesion protein 1)
别名 AOC3, VAP1, 2A6G3, Amine oxidase [copper-containing] 3, Amine oxidase copper-containing 3
计算分子量 85 kDa
观测分子量 85-90 kDa
GenBank蛋白编号BC050549
基因名称 AOC3
Gene ID (NCBI) 8639
RRIDAB_2882177
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDQ16853
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

AOC3(membrane primary amine oxidase) is also name as copper amine oxidase, HPAO, SSAO, VAP1 and belongs to the copper/topaquinone oxidase family. It is expressed on the surface of endothelial cells and is involved in leukocyte trafficking between blood and tissues under physiologic and pathologic conditions (PMID:17947691). AOC3 is highly expressed in adipocytes and smooth muscle cells and it can be N- and O-glycosylated (PMID:16046623). AOC3 has molecular mass of 70-90kD, and can exsit as a homodimer(165-185kD) and trimer(240~260kD) (PMID:10595925, 8625974).

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