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验证数据展示

  • IF Staining of A549 using CL488-68401

    IF Staining of A549 using CL488-68401

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using CoraLite® Plus 488 AMOTL2 antibody (CL488-68401, Clone: 2C4A4 ) at dilution of 1:200.

CoraLite® Plus 488-conjugated AMOTL2 Monoclonal antibody

AMOTL2 Monoclonal Antibody for IF/ICC
Cat No. CL488-68401

产品说明书

CloneNo. 2C4A4

宿主/亚型

Mouse / IgG1

种属反应性

Human

应用

IF/ICC

别名

AMOTL2, angiomotin like 2, Angiomotin like protein 2, KIAA0989, LCCP, Leman coiled coil protein

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inA549 cells
For other applications, we recommend the unconjugated version of this antibody, 68401-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-68401 targets AMOTL2 in IF/ICC applications and shows reactivity with Human samples.

经测试应用 IF/ICC Application Description
经测试反应性 Human
免疫原 AMOTL2 fusion protein Ag19971 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 angiomotin like 2
别名 AMOTL2, angiomotin like 2, Angiomotin like protein 2, KIAA0989, LCCP, Leman coiled coil protein
计算分子量 779 aa, 86 kDa
观测分子量100 kDa
GenBank蛋白编号BC011454
基因名称 AMOTL2
Gene ID (NCBI) 51421
RRIDAB_3673006
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ9Y2J4
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Angiomotin‑like 2 (AMOTL2) is a member of the motin family of angiostatin‑binding proteins. The motin family, also known as AMOTs, consists of three members: AMOT, AMOT-like 1 (AMOTL1) and AMOTL2. Members of the motin family are a type of adaptor proteins mainly distributed in the cytomembrane, cytoplasm or nucleus, and have a higher expression in the endothelial cells of capillaries and in larger vessels of the placenta. Human AmotL2 encodes two isoforms of a molecular mass of 100 kDa and 60 kDa.(PMID: 34036399,PMID: 25080976)

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 AMOTL2 antibody CL488-68401Download protocol
Standard Protocols
Click here to view our Standard Protocols