ALR Recombinant antibody

ALR Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 84972-4-RR

产品说明书

CloneNo. 242557G3

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, FC (Intra), ELISA

GFER, 242557G3, EC:1.8.3.2, ERV1, FAD-linked sulfhydryl oxidase ALR

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inA375 cells, A431 cells, HepG2 cells, mouse liver tissue, rat liver tissue
Positive FC (Intra) detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84972-4-RR targets ALR in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, FC (Intra), ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 ALR fusion protein Ag1840 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 growth factor, augmenter of liver regeneration
别名 GFER, 242557G3, EC:1.8.3.2, ERV1, FAD-linked sulfhydryl oxidase ALR
计算分子量 15 kDa, 23 kDa
观测分子量 23-25 kDa
GenBank蛋白编号BC028348
基因名称 GFER
Gene ID (NCBI) 2671
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP55789
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GFER (FAD-linked sulfhydryl oxidase) is also named as ALR, HERV1, HPO. It plays an important role in the disulfide relay system (DRS) in human mitochondria. The GFER gene codes for 2 distinct isoforms that are probably synthesized from the same mRNA with the use of different initiation codons. The long isoform (205 amino acids, 23/21 kD) is located mainly in the mitochondrial intermembrane space and exists under nonreducing and nondenaturing conditions as a homodimer and a heterodimer. The shorter isoform (125 amino acids, 15 kD), which lacks 80 amino acids at its N terminus compared to the longer isoform, is present predominantly in the nucleus (PMID: 19409522, 24880092, 21152698).

实验方案

Product Specific Protocols
WB protocol for ALR antibody 84972-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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