• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 84972-4-RR (same clone as 84972-4-PBS)

    WB analysis using 84972-4-RR (same clone as 84972-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84972-4-RR (GFER antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84972-4-PBS in a different storage buffer formulation.

  • FC experiment of HepG2 using 84972-4-RR (same clone as 84972-4-PBS)

    FC experiment of HepG2 using 84972-4-RR (same clone as 84972-4-PBS)

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug ALR Recombinant antibody (84972-4-RR, Clone:242557G3) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 84972-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84972-4-RR

    Affinity and Kinetic Characterization of 84972-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84972-4-RR against Human ALR were performed. The affinity constant is 0.23 nM.

ALR Recombinant antibody, PBS Only

ALR Uni-rAbTM Recombinant Antibody for WB, FC (Intra), Indirect ELISA
Cat No. 84972-4-PBS

产品说明书

CloneNo. 242557G3

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, FC (Intra), Indirect ELISA

别名

GFER, 242557G3, EC:1.8.3.2, ERV1, FAD-linked sulfhydryl oxidase ALR

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

产品信息

84972-4-PBS targets ALR in WB, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, FC (Intra), Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 ALR fusion protein Ag1840 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 growth factor, augmenter of liver regeneration
别名 GFER, 242557G3, EC:1.8.3.2, ERV1, FAD-linked sulfhydryl oxidase ALR
计算分子量 15 kDa, 23 kDa
观测分子量 23-25 kDa
GenBank蛋白编号BC028348
基因名称 GFER
Gene ID (NCBI) 2671
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP55789
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

GFER (FAD-linked sulfhydryl oxidase) is also named as ALR, HERV1, HPO. It plays an important role in the disulfide relay system (DRS) in human mitochondria. The GFER gene codes for 2 distinct isoforms that are probably synthesized from the same mRNA with the use of different initiation codons. The long isoform (205 amino acids, 23/21 kD) is located mainly in the mitochondrial intermembrane space and exists under nonreducing and nondenaturing conditions as a homodimer and a heterodimer. The shorter isoform (125 amino acids, 15 kD), which lacks 80 amino acids at its N terminus compared to the longer isoform, is present predominantly in the nucleus (PMID: 19409522, 24880092, 21152698).