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Host
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Species Reactivity
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Species Reactivity
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Conjugate
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Compatible Species
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Conjugate
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验证数据展示

  • WB analysis using 67689-1-Ig (same clone as 67689-1-PBS)

    WB analysis using 67689-1-Ig (same clone as 67689-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67689-1-Ig (ALPK1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67689-1-PBS in a different storage buffer formulation.

  • IHC staining of human heart using 67689-1-Ig (same clone as 67689-1-PBS)

    IHC staining of human heart using 67689-1-Ig (same clone as 67689-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 67689-1-Ig (ALPK1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67689-1-PBS in a different storage buffer formulation.

  • IF Staining of THP-1 using 67689-1-Ig (same clone as 67689-1-PBS)

    IF Staining of THP-1 using 67689-1-Ig (same clone as 67689-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using ALPK1 antibody (67689-1-Ig, Clone: 2B8B5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 67689-1-PBS in a different storage buffer formulation.

ALPK1 Monoclonal antibody, PBS Only

ALPK1 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 67689-1-PBS

产品说明书

CloneNo. 2B8B5

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

2B8B5, alpha kinase 1, Alpha protein kinase 1, Alpha-protein kinase 1, ALPK

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

67689-1-PBS targets ALPK1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human
免疫原 ALPK1 fusion protein Ag5320 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 alpha-kinase 1
别名 2B8B5, alpha kinase 1, Alpha protein kinase 1, Alpha-protein kinase 1, ALPK
计算分子量 139 kDa
观测分子量139 kDa
GenBank蛋白编号BC060780
基因名称 ALPK1
Gene ID (NCBI) 80216
RRIDAB_2882882
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ96QP1
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Alpha-protein kinase 1, also known as alpha-kinase 1 (ALPK1), is associated with chronic kidney disease (CKD), myocardial infarction, gout and type 2 diabetes mellitus (DM). ALPK1 mutant has been reported to cause severe defects in motor co‐ordination in mice. ALPK1 enhances the expression of proinflammatory cytokines including IL-1β, IL-8 and TGF-β1 in cultured human THP1 and human embryonic kidney 293 (HEK293) cells (PMID: 31557402, PMID: 26275947). ALPK1 short isoform (108 kDa) was highly expressed in brain, spinal cord, heart, lung, spleen, thymus, small intestine, skin and testis, while detectable inskeletal muscle and kidney. ALPK1 large isoform (130 kDa) appeared in heart, lung, thymus and skin (PMID: 21208416).