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验证数据展示

  • WB analysis using 67689-1-Ig

    WB analysis using 67689-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67689-1-Ig (ALPK1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of human heart using 67689-1-Ig

    IHC staining of human heart using 67689-1-Ig

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 67689-1-Ig (ALPK1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of THP-1 using 67689-1-Ig

    IF Staining of THP-1 using 67689-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using ALPK1 antibody (67689-1-Ig, Clone: 2B8B5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1).

ALPK1 Monoclonal antibody

ALPK1 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 67689-1-Ig

产品说明书

CloneNo. 2B8B5

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

WB, IHC, IF/ICC, ELISA

别名

Chromosome 4 kinase, ALPK, Alpha-protein kinase 1, Alpha protein kinase 1, alpha kinase 1

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inCOLO 320 cells, T-47D cells, A549 cells, U-87 MG cells, Daudi cells, THP-1 cells
Positive IHC detected inhuman heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inTHP-1 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67689-1-Ig targets ALPK1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 ALPK1 fusion protein Ag5320 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 alpha-kinase 1
别名 Chromosome 4 kinase, ALPK, Alpha-protein kinase 1, Alpha protein kinase 1, alpha kinase 1
计算分子量 139 kDa
观测分子量139 kDa
GenBank蛋白编号BC060780
基因名称 ALPK1
Gene ID (NCBI) 80216
RRIDAB_2882882
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ96QP1
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Alpha-protein kinase 1, also known as alpha-kinase 1 (ALPK1), is associated with chronic kidney disease (CKD), myocardial infarction, gout and type 2 diabetes mellitus (DM). ALPK1 mutant has been reported to cause severe defects in motor co‐ordination in mice. ALPK1 enhances the expression of proinflammatory cytokines including IL-1β, IL-8 and TGF-β1 in cultured human THP1 and human embryonic kidney 293 (HEK293) cells (PMID: 31557402, PMID: 26275947). ALPK1 short isoform (108 kDa) was highly expressed in brain, spinal cord, heart, lung, spleen, thymus, small intestine, skin and testis, while detectable inskeletal muscle and kidney. ALPK1 large isoform (130 kDa) appeared in heart, lung, thymus and skin (PMID: 21208416).

实验方案

Product Specific Protocols
WB protocol for ALPK1 antibody 67689-1-IgDownload protocol
IHC protocol for ALPK1 antibody 67689-1-IgDownload protocol
IF protocol for ALPK1 antibody 67689-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols