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Host
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Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
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Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 68396-1-Ig (same clone as 68396-1-PBS)

    WB analysis using 68396-1-Ig (same clone as 68396-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68396-1-Ig (ALDOB antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68396-1-PBS in a different storage buffer formulation.

  • WB analysis using 68396-1-Ig (same clone as 68396-1-PBS)

    WB analysis using 68396-1-Ig (same clone as 68396-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68396-1-Ig (ALDOB antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68396-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 68396-1-Ig (same clone as 68396-1-PBS)

    IF Staining of HepG2 using 68396-1-Ig (same clone as 68396-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using ALDOB antibody (68396-1-Ig, Clone: 3D2E4 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68396-1-PBS in a different storage buffer formulation.

ALDOB Monoclonal antibody, PBS Only

ALDOB Monoclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 68396-1-PBS

产品说明书

CloneNo. 3D2E4

宿主/亚型

Mouse / IgG2a

种属反应性

Human, mouse, rat, rabbit

应用

WB, IF/ICC, Indirect ELISA

别名

ALDB, ALDOB, Liver type aldolase

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy ALDOB Ready-To-Use IHC Kit. ALDOB primary antibody included.

产品信息

68396-1-PBS targets ALDOB in WB, IF/ICC, Indirect ELISA applications and shows reactivity with Human, mouse, rat, rabbit samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 Human, mouse, rat, rabbit
免疫原

CatNo: Ag12823

Product name: Recombinant human ALDOB protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-316 aa of BC029399

Sequence: MAHRFPALTQEQKKELSEIAQSIVANGKGILAADESVGTMGNRLQRIKVENTEENRRQFREILFSVDSSINQSIGGVILFHETLYQKDSQGKLFRNILKEKGIVVGIKLDQGGAPLAGTNKETTIQGLDGLSERCAQYKKDGVDFGKWRAVLRIADQCPSSLPIQENANALARYASICQQNGLVPIVEPEVIPDGDHDLEHCQYVTEKVLAAVYKALNDHHVYLEGTLLKPNMVTAGHACTKKYTPEQVAMATVTALHRTVPAAVPGICFLSGGMSEEDATLNLNAINLCPLPKPWKLSFSGVARLQTRRQPRRLL

种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 aldolase B, fructose-bisphosphate
别名 ALDB, ALDOB, Liver type aldolase
计算分子量 316 aa, 35 kDa
观测分子量 35-40 kDa
GenBank蛋白编号BC029399
基因名称 ALDOB
Gene ID (NCBI) 229
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP05062
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Fructose-1,6-bisphosphate aldolase is a glycolytic enzyme that catalyzes the reversible conversion of fructose-1,6-bisphosphate to glyceraldehyde 3-phosphate and dihydroxyacetone phosphate.Vertebrates have 3 aldolase isozymes, aldolase A (ALDOA), B (ALDOB), and C (ALDOC). Deficiency of this enzyme ALDOB causes an accumulation of fructose-1-phosphate after fructose intake, which results in toxic symptoms like vomiting, hypoglycemia, jaundice, elevated liver enzymes and hepatomegaly(PMID:22375183). This antibody may detect ALDOA.

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