• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

ALDOB Monoclonal antibody

ALDOB Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse, rat, rabbit

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

3D2E4

Cat No : 68396-1-Ig

Print datasheet

Synonyms

ALDB, ALDOB, Liver type aldolase

验证数据展示

  • WB analysis using 68396-1-Ig

    WB analysis using 68396-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68396-1-Ig (ALDOB antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis using 68396-1-Ig

    WB analysis using 68396-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68396-1-Ig (ALDOB antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inrat kidney tissue, human urine sample, rabbit liver tissue, rat liver tissue, mouse liver tissue, mouse kidney tissue
Planning an IHC experiment? We recommend our IHCeasy ALDOB Ready-To-Use IHC Kit. ALDOB primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68396-1-Ig targets ALDOB in WB, ELISA applications and shows reactivity with Human, mouse, rat, rabbit samples.

Tested Applications WB, ELISA
Tested Reactivity Human, mouse, rat, rabbit
Immunogen ALDOB fusion protein Ag12823 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name aldolase B, fructose-bisphosphate
Synonyms ALDB, ALDOB, Liver type aldolase
Calculated Molecular Weight 316 aa, 35 kDa
Observed Molecular Weight 35-40 kDa
GenBank Accession NumberBC029399
Gene Symbol ALDOB
Gene ID (NCBI) 229
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP05062
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Fructose-1,6-bisphosphate aldolase is a glycolytic enzyme that catalyzes the reversible conversion of fructose-1,6-bisphosphate to glyceraldehyde 3-phosphate and dihydroxyacetone phosphate.Vertebrates have 3 aldolase isozymes, aldolase A (ALDOA), B (ALDOB), and C (ALDOC). Deficiency of this enzyme ALDOB causes an accumulation of fructose-1-phosphate after fructose intake, which results in toxic symptoms like vomiting, hypoglycemia, jaundice, elevated liver enzymes and hepatomegaly(PMID:22375183). This antibody may detect ALDOA.

实验方案

Product Specific Protocols
WB protocol for ALDOB antibody 68396-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols