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验证数据展示

  • WB analysis using 27947-1-AP (same clone as 27947-1-PBS)

    WB analysis using 27947-1-AP (same clone as 27947-1-PBS)

    HeLa cells were subjected to Tris-Acetate gel system followed by western blot with 27947-1-AP (AKAP13 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27947-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 27947-1-AP (same clone as 27947-1-PBS)

    IHC staining of human breast cancer using 27947-1-AP (same clone as 27947-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27947-1-AP (AKAP13 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27947-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with AKAP13 antibody included
  • IHC staining of human breast cancer using 27947-1-AP (same clone as 27947-1-PBS)

    IHC staining of human breast cancer using 27947-1-AP (same clone as 27947-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27947-1-AP (AKAP13 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27947-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with AKAP13 antibody included

AKAP13 Polyclonal antibody, PBS Only

AKAP13 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 27947-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, Indirect ELISA

别名

A kinase anchor protein 13, AKAP 13, A-kinase anchor protein 13, ARHGEF13, BRX

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy AKAP13 Ready-To-Use IHC Kit. AKAP13 primary antibody included.

产品信息

27947-1-PBS targets AKAP13 in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag27486

Product name: Recombinant human AKAP13 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 359-587 aa of NM_001270546

Sequence: MIVEEENTDRSCRKKNKGVERKGEEVEPAPIVDSGTVSDQDSCLQSLPDCGVKGTEGLSSCGNRNEETGTKSSGMPTDQESLSSGDAVLQRDLVMEPGTAQYSSGGELGGISTTNVSTPDTAGEMEHGLMNPDATVWKNVLQGGESTKERFENSNIGTAGASDVHVTSKPVDKISVPNCAPAASSLDGNKPAESSLAFSNEETSTEKTAETETSRSREESADAPVDQNSV

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 A kinase (PRKA) anchor protein 13
别名 A kinase anchor protein 13, AKAP 13, A-kinase anchor protein 13, ARHGEF13, BRX
计算分子量 308 kDa
观测分子量308 kDa
GenBank蛋白编号NM_001270546
基因名称 AKAP13
Gene ID (NCBI) 11214
RRIDAB_2881017
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ12802
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The A-kinase anchor proteins (AKAPs) are a group of structurally diverse proteins which have the common function of binding to the regulatory subunit of protein kinase A (PKA) and confining the holoenzyme to discrete locations within the cell. This gene encodes a member of the AKAP family. Alternative splicing of this gene results in multiple transcript variants encoding different isoforms containing c-terminal dbl oncogene homology (DH) and pleckstrin homology (PH) domains. The DH domain is associated with guanine nucleotide exchange activation for the Rho/Rac family of small GTP binding proteins, resulting in the conversion of the inactive GTPase to the active form capable of transducing signals. The PH domain has multiple functions

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