验证数据展示
经过测试的应用
Positive WB detected in | HeLa cells |
Positive IHC detected in | human breast cancer tissue, human colon cancer tissue, human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:500-1:1000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
27947-1-AP targets AKAP13 in WB, IHC, ELISA applications and shows reactivity with human samples.
经测试应用 | WB, IHC, ELISA Application Description |
经测试反应性 | human |
免疫原 | AKAP13 fusion protein Ag27486 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | A kinase (PRKA) anchor protein 13 |
别名 | A kinase anchor protein 13, AKAP 13, A-kinase anchor protein 13, ARHGEF13, BRX |
计算分子量 | 308 kDa |
观测分子量 | 308 kDa |
GenBank蛋白编号 | NM_001270546 |
基因名称 | AKAP13 |
Gene ID (NCBI) | 11214 |
RRID | AB_2881017 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | Q12802 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The A-kinase anchor proteins (AKAPs) are a group of structurally diverse proteins which have the common function of binding to the regulatory subunit of protein kinase A (PKA) and confining the holoenzyme to discrete locations within the cell. This gene encodes a member of the AKAP family. Alternative splicing of this gene results in multiple transcript variants encoding different isoforms containing c-terminal dbl oncogene homology (DH) and pleckstrin homology (PH) domains. The DH domain is associated with guanine nucleotide exchange activation for the Rho/Rac family of small GTP binding proteins, resulting in the conversion of the inactive GTPase to the active form capable of transducing signals. The PH domain has multiple functions
实验方案
Product Specific Protocols | |
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WB protocol for AKAP13 antibody 27947-1-AP | Download protocol |
IHC protocol for AKAP13 antibody 27947-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |