AKAP13 Polyclonal antibody

AKAP13 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 27947-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

A kinase anchor protein 13, AKAP 13, A-kinase anchor protein 13, ARHGEF13, BRX

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inHeLa cells
Positive IHC detected inhuman breast cancer tissue, human colon cancer tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy AKAP13 Ready-To-Use IHC Kit. AKAP13 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27947-1-AP targets AKAP13 in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原 AKAP13 fusion protein Ag27486 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 A kinase (PRKA) anchor protein 13
别名 A kinase anchor protein 13, AKAP 13, A-kinase anchor protein 13, ARHGEF13, BRX
计算分子量 308 kDa
观测分子量308 kDa
GenBank蛋白编号NM_001270546
基因名称 AKAP13
Gene ID (NCBI) 11214
RRIDAB_2881017
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ12802
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The A-kinase anchor proteins (AKAPs) are a group of structurally diverse proteins which have the common function of binding to the regulatory subunit of protein kinase A (PKA) and confining the holoenzyme to discrete locations within the cell. This gene encodes a member of the AKAP family. Alternative splicing of this gene results in multiple transcript variants encoding different isoforms containing c-terminal dbl oncogene homology (DH) and pleckstrin homology (PH) domains. The DH domain is associated with guanine nucleotide exchange activation for the Rho/Rac family of small GTP binding proteins, resulting in the conversion of the inactive GTPase to the active form capable of transducing signals. The PH domain has multiple functions

实验方案

Product Specific Protocols
WB protocol for AKAP13 antibody 27947-1-APDownload protocol
IHC protocol for AKAP13 antibody 27947-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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