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验证数据展示

  • WB analysis using 68049-1-Ig (same clone as 68049-1-PBS)

    WB analysis using 68049-1-Ig (same clone as 68049-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68049-1-Ig (AIFM2/ FSP1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68049-1-PBS in a different storage buffer formulation.

  • WB analysis using 68049-1-Ig (same clone as 68049-1-PBS)

    WB analysis using 68049-1-Ig (same clone as 68049-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 68049-1-Ig (AIFM2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68049-1-PBS in a different storage buffer formulation.

  • WB analysis of K-562 using 68049-1-Ig (same clone as 68049-1-PBS)

    WB analysis of K-562 using 68049-1-Ig (same clone as 68049-1-PBS)

    K-562 cells were subjected to SDS PAGE followed by western blot with 68049-1-Ig (AIFM2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68049-1-PBS in a different storage buffer formulation.

  • Cytometric bead array sample test of MP51527-1

    Cytometric bead array sample test of MP51527-1

    Cytometric bead array sample test of MP51527-1, AIFM2 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68049-1-PBS. Detection antibody: 68049-2-PBS.

  • IP experiment of K-562 using 68049-1-Ig (same clone as 68049-1-PBS)

    IP experiment of K-562 using 68049-1-Ig (same clone as 68049-1-PBS)

    IP result of anti-AIFM2/ FSP1 (IP:68049-1-Ig, 4ug; Detection:68049-1-Ig 1:500) with K-562 cells lysate 1760 ug. This data was developed using the same antibody clone with 68049-1-PBS in a different storage buffer formulation.

  • FC experiment of K-562 using 68049-1-Ig (same clone as 68049-1-PBS)

    FC experiment of K-562 using 68049-1-Ig (same clone as 68049-1-PBS)

    1X10^6 K-562 cells were intracellularly stained with 0.5 ug Anti-Human AIFM2 (68049-1-Ig, Clone:1A2B2) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.5 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 68049-1-PBS in a different storage buffer formulation.

  • IF Staining of H9C2 using 68049-1-Ig (same clone as 68049-1-PBS)

    IF Staining of H9C2 using 68049-1-Ig (same clone as 68049-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed H9C2 cells using AIFM2/ FSP1 antibody (68049-1-Ig, Clone: 1A2B2 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 68049-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse liver using 68049-1-Ig (same clone as 68049-1-PBS)

    IHC staining of mouse liver using 68049-1-Ig (same clone as 68049-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 68049-1-Ig (AIFM2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68049-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with AIFM2 antibody included
  • IHC staining of mouse liver using 68049-1-Ig (same clone as 68049-1-PBS)

    IHC staining of mouse liver using 68049-1-Ig (same clone as 68049-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 68049-1-Ig (AIFM2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68049-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with AIFM2 antibody included
  • IF Staining of H9C2 using 68049-1-Ig (same clone as 68049-1-PBS)

    IF Staining of H9C2 using 68049-1-Ig (same clone as 68049-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed H9C2 cells using AIFM2/ FSP1 antibody (68049-1-Ig, Clone: 1A2B2 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red). This data was developed using the same antibody clone with 68049-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP51527-1

    Cytometric bead array standard curve of MP51527-1

    Cytometric bead array standard curve of MP51527-1, AIFM2 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68049-1-PBS. Detection antibody: 68049-2-PBS. Standard:Ag30516. Range: 1.563-200 ng/mL.

AIFM2/ FSP1 Monoclonal antibody, PBS Only (Capture)

AIFM2/ FSP1 Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, Cytometric bead array, Indirect ELISA
Cat No. 68049-1-PBS

产品说明书

CloneNo. 1A2B2

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, FC (Intra), IP, Cytometric bead array, Indirect ELISA

别名

AIFM2, AMID, FSP1, 1A2B2, AIFM 2

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy AIFM2 Ready-To-Use IHC Kit. AIFM2 primary antibody included.

产品信息

68049-1-PBS targets AIFM2/ FSP1 as part of a matched antibody pair:

MP51527-1: 68049-1-PBS capture and 68049-2-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IHC, IF/ICC, FC (Intra), IP, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag30516

Product name: Recombinant human AIFM2 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-373 aa of BC023601

Sequence: MGSQVSVESGALHVVIVGGGFGGIAAASQLQALNVPFMLVDMKDSFHHNVAALRASVETGFAKKTFISYSVTFKDNFRQGLVVGIDLKNQMVLLQGGEALPFSHLILATGSTGPFPGKFNEVSSQQAAIQAYEDMVRQVQRSRFIVVVGGGSAGVEMAAEIKTEYPEKEVTLIHSQVALADKELLPSVRQEVKEILLRKGVQLLLSERVSNLEELPLNEYREYIKVQTDKGTEVATNLVILCTGIKINSSAYRKAFESRLASSGALRVNEHLQVEGHSNVYAIGDCADVRTPKMAYLAGLHANIAVANIVNSVKQRPLQAYKPGALTFLLSMGRNDGVGQISGFYVGRLMVRLTKSRDLFVSTSWKTMRQSPP

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 apoptosis-inducing factor, mitochondrion-associated, 2
别名 AIFM2, AMID, FSP1, 1A2B2, AIFM 2
计算分子量 41 kDa
观测分子量41 kDa
GenBank蛋白编号BC023601
基因名称 AIFM2/ FSP1
Gene ID (NCBI) 84883
RRIDAB_2918791
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDQ9BRQ8
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The human AIFM2 protein (also known as FSP1 or AMID) is an apoptosis associated flavoprotein with a 6-hydroxy FAD cofactor. AIFM2 is a NAD(P)H-binding oxidoreductase with some sequence similarities to A1FM1 (formerly known as AIF, Apoptosis Inducing Factor), a mitochondrion-associated enzyme which relocates to the cell nucleus during apoptosis and is considered to be a key player in the progression of cell death.

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