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AIFM2/ FSP1 Monoclonal antibody

AIFM2/ FSP1 Monoclonal Antibody for FC, IF/ICC, IHC, IP, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse

Applications

FC, IF/ICC, IHC, IP, WB, ELISA

Conjugate

Unconjugated

CloneNo.

1A2B2

Cat No : 68049-1-Ig

Print datasheet

Synonyms

AIFM2, AIFM2/ FSP1, AMID, Apoptosis inducing factor 2, FSP1, p53 responsive gene 3 protein, PRG3, RP11 367H5.2

验证数据展示

  • WB analysis using 68049-1-Ig

    WB analysis using 68049-1-Ig

    HepG2 cells were subjected to SDS PAGE followed by western blot with 68049-1-Ig (AIFM2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of K-562 using 68049-1-Ig

    WB analysis of K-562 using 68049-1-Ig

    K-562 cells were subjected to SDS PAGE followed by western blot with 68049-1-Ig (AIFM2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IP experiment of K-562 using 68049-1-Ig

    IP experiment of K-562 using 68049-1-Ig

    IP result of anti-AIFM2/ FSP1 (IP:68049-1-Ig, 4ug; Detection:68049-1-Ig 1:500) with K-562 cells lysate 1760 ug.

  • FC experiment of K-562 using 68049-1-Ig

    FC experiment of K-562 using 68049-1-Ig

    1X10^6 K-562 cells were intracellularly stained with 0.5 ug Anti-Human AIFM2 (68049-1-Ig, Clone:1A2B2) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.5 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • IF Staining of H9C2 using 68049-1-Ig

    IF Staining of H9C2 using 68049-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed H9C2 cells using AIFM2/ FSP1 antibody (68049-1-Ig, Clone: 1A2B2 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

  • IHC staining of mouse liver using 68049-1-Ig

    IHC staining of mouse liver using 68049-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 68049-1-Ig (AIFM2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with AIFM2 antibody included
  • IHC staining of mouse liver using 68049-1-Ig

    IHC staining of mouse liver using 68049-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 68049-1-Ig (AIFM2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with AIFM2 antibody included

经过测试的应用

Positive WB detected inHepG2 cells, NCI-H1299 cells, A549 cells, HEK-293 cells, MCF-7 cells, K-562 cells, L02 cells
Positive IP detected inK-562 cells
Positive IHC detected inmouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inH9C2 cells
Positive FC detected inK-562 cells
Planning an IHC experiment? We recommend our IHCeasy AIFM2 Ready-To-Use IHC Kit. AIFM2 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)IF : 1:400-1:1600
Flow Cytometry (FC)FC : 0.50 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68049-1-Ig targets AIFM2/ FSP1 in WB, IP, IF, FC, IHC, ELISA applications and shows reactivity with Human, Mouse samples.

Tested Applications FC, IF/ICC, IHC, IP, WB, ELISA
Tested Reactivity Human, Mouse
Immunogen AIFM2/ FSP1 fusion protein Ag30516 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name apoptosis-inducing factor, mitochondrion-associated, 2
Synonyms AIFM2, AIFM2/ FSP1, AMID, Apoptosis inducing factor 2, FSP1, p53 responsive gene 3 protein, PRG3, RP11 367H5.2
Calculated Molecular Weight 41 kDa
Observed Molecular Weight41 kDa
GenBank Accession NumberBC023601
Gene Symbol AIFM2
Gene ID (NCBI) 84883
RRIDAB_2918791
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ9BRQ8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The human AIFM2 protein (also known as FSP1 or AMID) is an apoptosis associated flavoprotein with a 6-hydroxy FAD cofactor. AIFM2 is a NAD(P)H-binding oxidoreductase with some sequence similarities to A1FM1 (formerly known as AIF, Apoptosis Inducing Factor), a mitochondrion-associated enzyme which relocates to the cell nucleus during apoptosis and is considered to be a key player in the progression of cell death.

实验方案

Product Specific Protocols
WB protocol for AIFM2/ FSP1 antibody 68049-1-IgDownload protocol
IHC protocol for AIFM2/ FSP1 antibody 68049-1-IgDownload protocol
IF protocol for AIFM2/ FSP1 antibody 68049-1-IgDownload protocol
IP protocol for AIFM2/ FSP1 antibody 68049-1-IgDownload protocol
FC protocol for AIFM2/ FSP1 antibody 68049-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols