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验证数据展示

  • IHC staining of mouse testis using 83369-6-RR

    IHC staining of mouse testis using 83369-6-RR

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 83369-6-RR (AHR antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with AHR antibody included
  • IF Staining of HepG2 using 83369-6-RR

    IF Staining of HepG2 using 83369-6-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using AHR antibody (83369-6-RR, Clone: 240325G9 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red).

  • IF Staining of HeLa using 83369-6-RR

    IF Staining of HeLa using 83369-6-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using AHR antibody (83369-6-RR, Clone: 240325G9 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red).

  • IF Staining of HeLa using 83369-6-RR

    IF Staining of HeLa using 83369-6-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using AHR antibody (83369-6-RR, Clone: 240325G9 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red).

  • FC experiment of HeLa using 83369-6-RR

    FC experiment of HeLa using 83369-6-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug AHR Recombinant antibody (83369-6-RR, Clone:240325G9) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

  • FC experiment of MCF-7 using 83369-6-RR

    FC experiment of MCF-7 using 83369-6-RR

    1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug AHR Recombinant antibody (83369-6-RR, Clone:240325G9) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

AHR Recombinant monoclonal antibody

AHR Uni-rAb® Recombinant Antibody for IHC, IF/ICC, FC (Intra), ELISA
Cat No. 83369-6-RR

产品说明书

CloneNo. 240325G9

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IHC, IF/ICC, FC (Intra), ELISA

别名

240325G9, Ah receptor, bHLHe76, Class E basic helix-loop-helix protein 76

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells, HepG2 cells
Positive FC (Intra) detected inMCF-7 cells, HeLa cells
Planning an IHC experiment? We recommend our IHCeasy AHR Ready-To-Use IHC Kit. AHR primary antibody included.

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83369-6-RR targets AHR in IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 IHC, IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag12193

Product name: Recombinant human AHR protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-387 aa of BC070080

Sequence: MNSSSANITYASRKRRKPVQKTVKPIPAEGIKSNPSKRHRDRLNTELDRLASLLPFPQDVINKLDKLSVLRLSVSYLRAKSFFDVALKSSPTERNGGQDNCRAANFREGLNLQEGEFLLQALNGFVLVVTTDALVFYASSTIQDYLGFQQSDVIHQSVYELIHTEDRAEFQRQLHWALNPSQCTESGQGIEEATGLPQTVVCYNPDQIPPENSPLMERCFICRLRCLLDNSSGFLAMNFQGKLKYLHGQKKKGKDGSILPPQLALFAIATPLQPPSILEIRTKNFIFRTKHKLDFTPIGCDAKGRIVLGYTEAELCTRGSGYQFIHAADMLYCAESHIRMIKTGESGMIVFRLLTKNNRWTWVQSNARLLYKNGRPDYIIVTQRPLT

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 aryl hydrocarbon receptor
别名 240325G9, Ah receptor, bHLHe76, Class E basic helix-loop-helix protein 76
计算分子量 848 aa, 96 kDa
GenBank蛋白编号BC070080
基因名称 AHR
Gene ID (NCBI) 196
RRIDAB_3671023
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDP35869
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The aryl hydrocarbon receptor (AhR) is a ligand-activated transcription factor that has been largely regarded as a mediator of xenobiotic metabolism (PMID:18483242). It plays a part role in physiologic activities, including attenuation of the acute phase response, cytokine signaling, T helper (TH) 17 immune cell differentiation, modulation of NF-κB activity, and regulation of hormonal signaling (PMID:20423157,18540824). It also mediates transcription factor sequestering away from a gene promoter or tethering of the AhR to a transcription factor on a promoter. AHR calculated molecular masses differ by <10%, compared with the apparent molecular masses predicted from SDS-PAGE for the two receptors (105 and 95 kDa, respectively). (PMID: 8246913)

实验方案

Product Specific Protocols
IF protocol for AHR antibody 83369-6-RRDownload protocol
IHC protocol for AHR antibody 83369-6-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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