验证数据展示
经过测试的应用
| Positive IF/ICC detected in | A431 cells |
Planning an IHC experiment? We recommend our IHCeasy MTDH Ready-To-Use IHC Kit. MTDH primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 84686-4-PBS
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-84686-4 targets AEG-1/MTDH in IF/ICC applications and shows reactivity with human samples.
| 经测试应用 | IF/ICC Application Description |
| 经测试反应性 | human |
| 免疫原 | AEG-1/MTDH fusion protein Ag4840 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | metadherin |
| 别名 | AEG-1, MTDH, 242110B5, 3D3/LYRIC, AEG 1 |
| 计算分子量 | 582 aa, 64 kDa |
| 观测分子量 | 75 kDa |
| GenBank蛋白编号 | BC045642 |
| 基因名称 | AEG-1/MTDH |
| Gene ID (NCBI) | 92140 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q86UE4 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
MTDH, also known as AEG-1, is a single-pass transmembrane protein with its gene located at chromosome 8q22 , the abnormalities of which have been identified in several tumor types. MTDH is overexpressed in a number of malignancies, such as breast cancer, malignant glioma, and neuroblastoma , and its overexpression is associated with poor clinical outcome. MTDH plays an important role in the regulation of carcinogenesis and tumor progression.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 AEG-1/MTDH antibody CL488-84686-4 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |